LOCUS       sequence-93939-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  doxycycline inducible multicistronic lentiviral gene expression 
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  TITLE     pUltra plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUltra-dox
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(36..69)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(91..144)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(166..222)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(315..338)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(513..532)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgag tcgacataac ttcgtatagt acacattata
       61 cgaagttatg aattctgatc acacgctagc tgggccggga ttttcctcca cgtccccgca
      121 tgttagtaga cttcccctgc cctcgccgga gccggatcct ctagaaggcc cgggattctc
      181 ctccacgtca cctgcttgtt tgagtagtga gaagtttgtt gctccagatc ccttgtacag
      241 ctcgtccatg ccgtacagga acaggtggtg gcggccctcg gagcgctcgt actgttccac
      301 gatggtgtag tcctcgttgt gggaggtgat gtccagcttg gtgtccacgt agtagtagcc
      361 gggcagttgc acgggcttct tggccatgta gatggtcttg aactccacca ggtagtggcc
      421 gccgtccttc agcttcaggg cctggtggat ctcgcccttc agcacgccgt cgcgggggta
      481 caggcgctcg gtggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcggg
      541 ggggaagttg gtgccgcgca tcttcacctt gtagatcagc gtgccgtcct gcagggagga
      601 gtcctgggtc acggtcacca gaccgccgtc ctcgaagttc atcacgcgct cccacttgaa
      661 gccctcgggg aaggacagct tcttgtaatc ggggatgtcg gcggg
//
