LOCUS       sequence-93960-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for baculovirus expression of CAMSAP2 CC+CKK in Sf9 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pFasBac+GFP-CAMSAP2 CC+CKK
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..37)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(59..76)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(143..234)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(201..218)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(586..591,592..608,609..614)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ctcctggatc catggcgccc tgaaaataca ggttttcggt cgttgggata tcgtaatcgt
       61 gatggtgatg gtgatggtag tacgacatgg tttcggaccg agatccgcgc ccgatggtgg
      121 gacggtatga ataatccgga atatttatag gtttttttat tacaaaactg ttacgaaaac
      181 agtaaaatac ttatttattt gcgagatggt tatcatttta attatctcca tgatctatta
      241 atattccgga gtatacgtag ccaaccacta gaactatagc tagagtcctg ggcgaacaaa
      301 cgatgctcgc cttccagaaa accgaggatg cgaaccactt catccggggt cagcaccacc
      361 ggcaagcgcc gcgacggccg aggtcttccg atctcctgaa gccagggcag atccgtgcac
      421 agcaccttgc cgtagaagaa cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa
      481 accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt
      541 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt
      601 tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
      661 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      721 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
      781 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcg
//
