LOCUS       sequence-93965-e        7531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid for assessing neuronal 
            activity-regulated enhancer function.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7531)
  TITLE     Nued2 luciferase reporter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGL4.11 Nued2
FEATURES             Location/Qualifiers
     source          1..7531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3255..3264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3261..4910
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             4914..5033
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    5085..5206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5096..5115)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5150..5169
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5454..5471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5625..6213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5705..5724)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6413..7204,7205..7273))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    7378..7426
                     /note="synthetic polyadenylation signal"
     misc_feature    7440..7531
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7480..7499
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcttcctggc ttgtagtgac ctcgtctatt
       61 gctagaaatc agaaggtacc actgagcctt catttgtttg gtgaaatcat ggagttacat
      121 taacaagccg tttccccaaa ctatggctct ctacccactt gtaatacacc cgtgcacagg
      181 aacattttct gaaagtcaat gtttcctatc acttgctcca aaattaaacc agagatacat
      241 cattcaggaa gaagccagat gcactgaaga aagaaaaacc tgctttatca gaaggggcaa
      301 gcttctctca catcccatag caatttaaat tcatttgccg gaatgttaaa tacaaccagt
      361 cttttagttg ggtcccttca cataaacacc aagttagcat aaatgcaaaa tagttcacat
      421 gattaaatct ttataacacc ataatttatc ttatgtacac acacacacac agagaaagag
      481 agagagagag agagagagag agatctttta cttgctgata cttctacatt ttttctggag
      541 ttctcaactc tcatcatctc tctaaggaag ctggcttgta ttgccccaga ctcttagtgt
      601 atttagttct aaaggatagt atacagaata aagggaaatt ttaattttgt ttttaatagg
      661 acctatataa ttcaggccta gctctggtgg agagaagggg aggagaggtc cattagcatt
      721 tattgtgcac ctgctttatg ctaaacatgt ctggaaacag tgtctcacac aacctgcagg
      781 acacttaatt aagcgcgttt aaacgtgtgg cgcgccagct agtaacagtt ggcattggga
      841 ctagaaggcc tcattcctaa accagcagat ttttccattg taccaggaaa acaaaactgc
      901 aaatagcata agtcctctga ttttactgtc tccagagaaa atcatatcct caatgcctgg
      961 gtctttccat ctggaatttg gcaactctgc tgatcccttc tcatagtggg aactcttgta
     1021 ggaagtctaa gagcaattat tttggggata tatgctattt ttatgtcttc ttgtacatca
     1081 ccttctttgg tcaggaggaa attagaattc tggaaatacg tttaattatt aaaaggacaa
     1141 gccttggctt caagtagttg caaatatgaa ttattagtcc atgtcatgaa agggctggag
     1201 aactacaaag aaacaatgat aatctatttg tcacacccaa ttaagatctg ccctggcctg
     1261 ggagtaaaag agcattaaat ctgtgagtgg taacttcaag cagcatgtct atatttgggt
     1321 aattctgaac atgcctggat ttggtacctt acagcatcct ctaaaaatgt actactttag
     1381 aaagtgtttt gagattttgt tgccttcgtg attgcagttt gtgtgtgtgt gtgtgtgtgg
     1441 ggggtgttat ttgagctttt gagtttccat gtagccaaag aaagaaagtc taggaagaaa
     1501 tggtagactc aaggtcaaat tcaacaaggg gaaacttctt tgtggtgagt ctagacaaca
     1561 aagccattag cccagtgttc acccccctgc ctacacccta ccttttccat tgatgaatat
     1621 tcttacctga gccatcctcg cttctgagtc ctgctctggg tcctgtggag actgaatatc
     1681 ctgtctgtag tttttaatcc ttgaagagat ttttcatgaa tagaactttc cttataattt
     1741 agcaacactg tccttgaaaa cccccacaac tatttacata acagaagcta ttgtttgaag
     1801 tgcaaaacag gtcagaattt ggttcccaga gattaatcac tgcagtctat tttgtattat
     1861 cataataatt tcatttattt tgatacaatt tgttttcaga atcaacccca gcttctttgc
     1921 acaagtgaca aatccatttg tttctggtag aatgggtaat gtgattaatg accttgcaac
     1981 aggatttctt taaaaagggg gaactttttt tttttcattt ttaattcaca gtaaaccagc
     2041 tgagtctgtt aagtgtgtaa ctgttctgaa cttgtaagcc atgggtaaga ttttgaaata
     2101 ccccctccca atcttttgcg gggtttctgg gtattaatat taggctttct ccaagaatct
     2161 gatgatacta gtctccaggt gtaaagtgga cttgttggat tcccgcatta aaaagaacgg
     2221 ggccccagtc tttgccatgg ccccatcatg tttattccgg ctggatgttc tcatcaaacc
     2281 gaggtcatgc gaaataaata aggtcatgcc tagaaggata ttgtccccgc acaagaacct
     2341 tcgagtgaac tcgtgactac tgcctcctgg tcttctgtgt gggcatcccc ttctttcttg
     2401 cagcatccag gacccacttc tgtccctgct ggtctgtttc tttctttctt tctttctttc
     2461 tttctttctt tcttgttttc ttccgttttt ccccttgagt tctctggggt tgggcctggg
     2521 attttgggta cgcctacaga ctgtccgcct ctgcaatcga ccgtcgtgct tcccgcgcca
     2581 gaacaaggaa gcccacgcga gccgcgtagg tggctcgcgt ggaagacccg ggttcgagtc
     2641 ccgctgggag aattcctcct aggcaagtgg agtcatctcg gtcaggattt ctcagccgga
     2701 agccgcgcca acacctctca tcgtgggcgg caagttgtcc ccggggcttc tttgaccgtg
     2761 cggggccagc cgacgctttc tctgaccgtt tccgaagccc agtttccttt cctaaggagc
     2821 cttggggatc ctgcactcgc gtctctgaaa cccaacccac gacagaaccc agccgcctgg
     2881 gccagcttag caatgcgccc gcgccccgcc ctccgcgtga cgcgaacgcc ggcggaccaa
     2941 tcagagagcg cggcgcgagc cccacgcgcc tgtggcccca ccctccaagc ttaagaaagg
     3001 gcgcgcgggc agagcccggg ccagaacgcc gcgcaaagcg gagagcgggt ttttgcgacg
     3061 gcaggcggcg cttgtgctgc cgtttccgtg ctgcacgcgg ttccctcgag tgccgcgctc
     3121 ggacagacgt agccggccgc gaaggcgccc agacggcaaa ccagcgaccc atgctgaagt
     3181 gagcacacag gtcagcgagg atatcaagat ctggcctcgg cggccaagct tggcaatccg
     3241 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
     3301 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
     3361 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
     3421 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
     3481 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
     3541 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
     3601 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
     3661 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
     3721 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
     3781 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
     3841 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
     3901 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
     3961 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
     4021 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
     4081 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
     4141 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
     4201 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
     4261 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
     4321 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
     4381 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
     4441 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
     4501 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
     4561 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
     4621 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
     4681 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
     4741 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
     4801 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
     4861 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg aattctcacg
     4921 gcttccctcc cgaggtggag gagcaggccg ccggcaccct gcccatgagc tgcgcccagg
     4981 agagcggcat ggatagacac cctgctgctt gcgccagcgc caggatcaac gtctaaggcc
     5041 gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     5101 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     5161 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5221 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     5281 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
     5341 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     5401 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     5461 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5521 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5581 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5641 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5701 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5761 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5821 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5881 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5941 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6001 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6061 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6121 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6181 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6241 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6301 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6361 tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt
     6421 gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct
     6481 gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag
     6541 caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag
     6601 cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga
     6661 gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg
     6721 ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg
     6781 gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct
     6841 ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa
     6901 tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg
     6961 gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc
     7021 cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg
     7081 ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga
     7141 tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg
     7201 ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat
     7261 gttggatgct catactcgtc ctttttcaat attattgaag catttatcag ggttactagt
     7321 acgtctctca aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat
     7381 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     7441 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     7501 agtgcaagtg caggtgccag aacatttctc t
//
