LOCUS       sequence-93966-e       13861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV 
            promoter). Also suitable for in vitro transcription (T7 promoter) 
            for injecting mRNA into oocytes. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13861)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 13861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-ELYS-emerin-EGFP-polyA
FEATURES             Location/Qualifiers
     source          1..13861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4893..4901
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      8427..8436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8433..8435,8436..8438,8439..9152)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             8433..9149
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(8478..8499)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8739..8758)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9086..9107
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9364..9393
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9409..9426
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9449..9466)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9455..9679
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9725..10153
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9812..9831)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10022..10043
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10162..10182)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10167..10497
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10348..10483
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10410..10429
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10564..11358
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10618..10637)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11228..11247
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11534..11655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11571..11590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11625..11644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11704..11720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11704..11720)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11717..11739)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11728..11744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11752..11758,11759..11776,11777..11782))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11797..11818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11935..11952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12106..12694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12186..12205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12865..13656,13657..13725))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13488..13507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13726..13830)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttcga attcatgcaa gacttgacag ctcaagtgac tagtgatctc ctgcatttcc
      961 cagaagtgac tattgaagct cttggagaag atgagataac attagagtcc gtgcttcgtg
     1021 gaaagtttgc tgcagggaaa aatggactag catgcttagc ttgtggtcca caacttgaag
     1081 ttgtaaactc cttaacagga gagcggttat ctgcatatag attcagtgga gtaaatgaac
     1141 agcctcctgt agtccttgca gtgaaagaat tctcttggca taagaggact ggattgttaa
     1201 taggattgga agaagcagat gggagtgttc tttgtcttta tgaccttggt atatcaagag
     1261 tggtcaaagc agttgttctt cctggaaggg taacagctat cgagcctata attaaccatg
     1321 gaggagccag tgcgagtacc cagcatttac atccaagtct ccggtggctt tttggcgtgg
     1381 ccgctgtggt gactgatgtt ggacagatcc ttcttattga cctgtgtttg gatgacttgt
     1441 cctgcagtca gaatgaagtt gaggcatcag accttgaagt tatcactggt atcccagctg
     1501 aagtaccaca catcagagag agagtgatga gagaggggcg ccacctgtgc ttccagttag
     1561 taagcccatt gggagtagcc atttctactc tgagttacat caacaggaca aatcagcttg
     1621 ctgtgggttt ttctgatggc tacttagcac tttggaacat gaaaagcatg aaaagagaat
     1681 actatacaca gttggaaggt ggaagggttc ctgtccatgc agttgccttt caagagcctg
     1741 agaatgatcc tcgtaactgc tgttatttat gggctgttca gtccacacaa gatagtgaag
     1801 gggatgtttt gagtttgcat ctgcttcagc tggcttttgg tgatagaaaa tgtttggcat
     1861 cagggcaaat tttatatgag ggattagaat actgcgaaga aagatataca ctggatctag
     1921 caggtggcac gttcccctta aggggacaaa ctagtaatac caaattgttg ggatgccaga
     1981 gtatagagag atttccatct catggagaca gagaagaaag tatgagagaa gctctgtctc
     2041 ccgataccag cgtttctgtc tttacctggc aagtgaatat atatggacag ggaaagcctt
     2101 ctgtgtattt agggctattt gacataaatc gttggtatca tgcacaaatg cccgattctt
     2161 taagatcagg agaatctctg cataattgct cttattttgc gttgtggtca ttggattcgg
     2221 ttgtaagtag gacttctcca catcacatct tggacatact agtacatgag aggagtttaa
     2281 accgaggggt tcctccttcc tacccacctc cagagcaatt ttttaaccca agtactttta
     2341 attttgatgc cacttgtttg ttagactctg gagttatcca tgtaacttgt gctggatttc
     2401 agaaggagac tttgacattt ttaaagaaat caggaccaac tcttaatgaa gtcattcctg
     2461 atagttataa tcgatgtctt gttgctggtc tcctctcacc aagacttatt gatattcagc
     2521 cttccagttt aagtcaagaa gaacaattag aagctatatt gtcagcagca attcagacaa
     2581 gttccttggg acttttgact ggttacatca gaacatggat aatagaagaa caaccaaatt
     2641 ctgctgctaa tctacgattt gttcttgagt ggacatggaa taaagtggtt ctcacaaaag
     2701 aagagtttga taggctttgt gtgccgctgt ttgacggttc gtgtcgtttt attgacccac
     2761 agactattca gtctatccag cagtgccatt tactgcttag caaccttagt acagtcttaa
     2821 gctgttttgc aatggaggcc cagggtatca ctgagagagg actggtggac ttgagcaaca
     2881 agcacatggt cacccagctt ctctgtcagt acgcacacat ggttctgtgg ttctgccact
     2941 cgggacttct gcccgaaggc ttagatgatg ctctgcagct gtcaagacta cgctacaact
     3001 accctgtaat tcagaactac tatacaagtc gtcggcagaa gtctgagcgc tcacccagag
     3061 ggaagtggaa ccacgactgc ttgatgattg atggattagt ctctcaacta ggagatgaag
     3121 ttgagaagtt gtggaagcgg gacgaaggtg gcacaggaag ataccctcct gctagcatcc
     3181 acgcattact tgatatatat ttattagaca acattaccga agcaagcaaa catgctatta
     3241 ccatttattt gctgcttgat attatgtatt cctttccaaa taaaacggat acccccattg
     3301 aatctttccc aactgccttt gctatttctt ggggccaagt taagctagtt caaggatttt
     3361 ggctactaga tcataatgac tatgagaatg gtttagacct tctgtttcac ccagttactg
     3421 caaagcctgc atcgtggcaa cattcaaaga taattgaagc ttttatgagt cagggagagc
     3481 acaaacaggc tctccggtat cttcagacaa tgaagccaac agtgtccagt agcaatgaag
     3541 ttatccttca cctcactgtt ctacttttta atagatgcat ggttgaggcc tggaacttac
     3601 tgcgacagaa ttcaaacaga gtaaatatag aggaattatt aaagcacgct tatgaagttt
     3661 gtcaggagat gggcttaatg gaggatttac tgaagctgcc atttacaaac actgagcagg
     3721 aatgcttagt gaaattttta cagtccagta ccagtgttga gaatcatgaa ttccttctag
     3781 ttcaccattt acagcgtgcc aattatattt ctgccttgaa actaaaccag attctgaaga
     3841 ataatctcat gagtgatcgt gaccctcgat tgcgggaaag atcggtgact cgaaattcta
     3901 tattagacca gtatgggaaa atcctaccta gagtccagag aaagttagct gttgagcgag
     3961 ctaagcctta ccacctgtcg acatcctcag tttttcatga agtttctaga cccaaaccgt
     4021 tatcggcatt tccaaagaaa gctataactg gaacagtgtt aacccgatct acgttcatca
     4081 gcaatgtttt atctaaaatt ggagaggtgt gggcaagtca tgaacctaga aatggcgtct
     4141 cactttttaa cagtcctaaa acagaacagc catctcctgt agtacactct ttcccacacc
     4201 cagagcttcc tgaggcgttt gttggaactc caatttcaaa tacatcccag agaatttcta
     4261 gattactgga tttggttgtc catcctgtac cccagccttc tcagtgtttg gagtttattc
     4321 aacaaagtcc cacaagatct cctttgtgtc tgctgtccag ttcgttacca ttaagttcac
     4381 agtttaaaag gccacatcag aatacctcca ggccttcaga gttgctttta cttgagactc
     4441 ctctcatagt taagaaagct aaatctttgg ctctgtcagc cacgtcttct ggatttgccg
     4501 agtttactcc tccatccatc cttaggtctg gtcttcgaac aacaccttta gcatctccct
     4561 ctttgtcacc tggaagatct ctcactccgc ctttcagagt taaagaaact aggatttcat
     4621 tcatggaaga aggcatgaat acacactgga ctgatagagc tacagatgac cgaaatacaa
     4681 aagcgtttgt tagcacatct ttccataaat gtggacttcc agcagaaact gagtggatga
     4741 agaccagtga taagaataca tattttcctc tggatgtccc tgcaaagggc cctcagaaag
     4801 tggtggcaga gtcactggct acccattcag gaaggctgga gaaactggat gtgagcaaag
     4861 aagacagcac agcttccacc aggtcagacc agacctcctt agagtatcat gacgcaccat
     4921 caccagaaga cttggaaggt gctgtttttg tgtctcccaa gccagcatct tcctccactg
     4981 aactaactac taattcaact ctacaaacag agagggataa tgataaagat gcgtttaagt
     5041 cagaaggtac tccttcaccc gtgaagaaac aaataggcac gggagacgct gcagtggaag
     5101 cattttcaga actgagtcgc ttagaccctg ttgaaagagc tgaagcttct tttggtgtgt
     5161 cgtcagtctg tgaaggggaa acctccactt caaactccaa gacgtcagtt ctggatggaa
     5221 tcgtgcctat tgagagccga acctccatac ttacagcaga ccacaaagag tctgtggcca
     5281 acacggttgc agatgttgaa agctctgggt ccaccagctc caagtgcccg gttacctctg
     5341 aacgcagcct cggccaaaaa ctaacattaa acttaaaaga agatgaaata gaagctcatg
     5401 taccaaagga gaacgttggt ttaccagaag aaagccctcg aatttctgct gctccttctg
     5461 atactcacga gattcatcta attggatgtg aaaatcttga agttcaaaat tcagaagagg
     5521 aggccaagaa tctttcattt gatgagttgt atcccttagg ggcagagaaa cttgagtata
     5581 atctcagtac tattgagcag cagttttgtg acttgcctga tgacaaagac tctgctgaat
     5641 gtgatgctgc tgaagtagac ggggaacttt ttgtggccca gagcaacttt accctgattt
     5701 tagaaggtga agaaggagaa gctgaggcaa gtgactctgc agcacctaat atgttaccaa
     5761 aagcgaccaa ggaaaaacct gtgagccaca gggaacccca taatcaggag cgcgttacag
     5821 atttgccatc tgctgtgact gctgaccaag aatcccacaa ggtagagact ttaccgtatg
     5881 tgcctgaacc ggttaaagtg gcaattgcag aaaatctgtt ggatgtaatt aaagacacca
     5941 gaagtaagga agcaactccc gtggcagcag gtgaggctgg tgatgaggac ggagcagtga
     6001 tagtcccaaa ggctgcacat tcgtccaggc tgacaaactc tacaccgaag actgttaagg
     6061 aaccacgtgc agagactgta aatacccgcc agagtgatga catggtttct tctagaactc
     6121 tcacaagaag gcagcatgcc ctaagcctga atgtcacatc agaacaagag ccttcagcag
     6181 ttgccactcc taagaagaga actagaaaaa ttaaagaaac tcctgagtct tctgaaagga
     6241 cctgttctga cctaaaagta gcacctgaga accaactgac agctcagagt cctcccgctc
     6301 ctaggagagg aaagaagaag gacgttagcc aaggcacact gccaagttct ggtgctgtgg
     6361 agccggaacc tcagggtacg ccgggaagac tgaggctgag aacgcagcca cccgagccag
     6421 cagctgaaga aactccttct agaacaaaag tcaggctttc atctgttaga aagggaaccc
     6481 ctagaagact taagaagtct gtagaaaatg ggcaaagtac agaaattcta gatgatctca
     6541 aagggagtga ggcagcaagt catgacggga ctgtcacaga gctgaggaat gccaagctag
     6601 aagatactca gaatatggag tataaacaag atgaacacag tgaccagcaa ctgcctctaa
     6661 aacgaaagag ggtcagagag agagaagtta gtgtgtcaag tgtgacagaa gagccaaagc
     6721 ttgactcatc ccagttgcct cttcagacag gactcgatgt acctgccacc cctaggaaac
     6781 gtggtagacc caggaaggta gttcccttag aagctgatgg tggcacaact ggtaaggaac
     6841 agacaagtcc tcagaagaaa gatgttccgg ttgtccggag atctacacgg aacaccccag
     6901 ctagaaatgt gagtacttta gaaaaatcag ttttagtgcc aaataaggaa gctgctctag
     6961 tggtgacatc taagaggaga cctacaaaga agtctgcaga ggaaagctca aaagatccat
     7021 cagcggcagt ctcagacttg gcgggtggag cagcccacac agagtccgct gaccgaaggg
     7081 acggactgct tgccgccgct gctctcacgc catctgccca gggcacaagg actaggtcta
     7141 gaaggaccat gttgttgacg gacatttctg aacccaaaac tgagccttta tttcctcctc
     7201 cttcagtgaa ggttccaaag aaaaaatcaa aagctgagaa catggaggcc gcagcccagc
     7261 tgaaagaatt ggtgtcagat atatcttctc agtttgttgt ttcccctcct gccttgagaa
     7321 ccaggcagaa aagtatatcc aatacttcca agcttctagg tgaactggag agtgacccta
     7381 aaccattaga gatcatagaa caaaaaccaa aaagaagcag gactgtgaag acaagagcaa
     7441 gcagaaacac aggaaaagga agttcttggt cacctcctcc tgtagaaatt aagctggttt
     7501 ctcccttggc gagtccagtg gatgaaataa agaccggcaa gccaagaaaa actgcagaaa
     7561 tagcaggaaa aactcttgga aggggcagaa agaagccatc ttcttttcca aagcaaattt
     7621 tacgcaggaa aatgctggac gactatgcgg ttttgtccga tactgagctg gccgcagtgc
     7681 tacgccagta caacatccct catgggccta ttgtgggttc cactcgcaag ctctacgaaa
     7741 agaaaatctt cgagtacgag acccagagaa ggagactctt accccccaac tcgtcatctt
     7801 cttcattctc ctatcagttc tcagacttgg attcagccgc cgtggactca gacatgtatg
     7861 atctgcccaa aaaagaagac gccttacttt accagagcaa ggactataat gatgactact
     7921 atgaggagag ttatttgacc accaagacat acggggagcc tgagtctgtg ggcatgtcca
     7981 agagcttccg ccagccgggg acctcacttg tagatgctga taccttccat caccaggtgc
     8041 gtgatgacat tttctcttct ttagaagagg aaggcaagga tagggagcgc ctcatctatg
     8101 gccaagacag tgcctaccag agcatcgcac actaccgccc catttccaat gtctccagga
     8161 gctctctggg cttatcatat tatcctacat cctccacctc ttctgtgtcc tcctcttcat
     8221 cttctccctc ttcatggctt acccgacgtg ccattcggcc agagaagcag gcccctgctg
     8281 ctgctctggg ccaggatcga caggtcccac tctggggcca gctgttactc ttcctggtct
     8341 ttgctgcctt tctgctcttt gtttactatt ccatacaagc tgaagaaggc aaccccttct
     8401 ggatggatcc cagggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     8461 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     8521 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     8581 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     8641 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     8701 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     8761 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     8821 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     8881 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     8941 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     9001 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     9061 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     9121 tcactcacgg catggacgag ctgtacaagt aagcggccgc tcgactatgg gcaccaaaga
     9181 acctgtaaac gttatctttt taaattgaat gtgcacaaat aaaagtttgg aaaagaaaaa
     9241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     9301 aaaaaaaaaa aaaaaaaata cgcatcatac gctgtggcgt acctcgagtc tagagggccc
     9361 ttcgaacaaa aactcatctc agaagaggat ctgaatatgc ataccggtca tcatcaccat
     9421 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     9481 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     9541 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     9601 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     9661 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     9721 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9781 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9841 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     9901 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9961 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    10021 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    10081 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    10141 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
    10201 ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
    10261 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    10321 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    10381 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
    10441 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    10501 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
    10561 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
    10621 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
    10681 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
    10741 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
    10801 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
    10861 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
    10921 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
    10981 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
    11041 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
    11101 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
    11161 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
    11221 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
    11281 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
    11341 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc
    11401 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    11461 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    11521 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    11581 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    11641 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    11701 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    11761 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    11821 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    11881 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    11941 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    12001 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    12061 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    12121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    12181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    12241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    12301 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    12361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    12421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    12481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    12541 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    12601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    12661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    12721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    12781 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    12841 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    12901 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    12961 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    13021 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    13081 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    13141 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    13201 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    13261 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    13321 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    13381 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    13441 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    13501 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    13561 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    13621 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    13681 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    13741 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    13801 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    13861 c
//
