LOCUS       sequence-9397-ex       10955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 10955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pDESTmycTNRC6C
FEATURES             Location/Qualifiers
     source          1..10955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        319..522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     473..493
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     519..543
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             639..668
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    678..702
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3008..3025
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(5825..5849)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5881..5900)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5882..5900)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5911..5928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5911..5927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5920..5942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    6332..6466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6369..6388)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6423..6442
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6592..7047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6679..6698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6889..6910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7166..7523
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7166..7357
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7189..7209)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7374..7509
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7574..8368
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7628..7647)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8238..8257
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8432..8480
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8521..8540)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8640..8662
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8700..8718)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8786..8890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8891..8959,8960..9751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9109..9128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9922..10510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10411..10430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10664..10681
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cattagatgc atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
       61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      601 agcctccgga ctctagccta ggccgcggta ccaccatgga acaaaaactt atttctgaag
      661 aagatctgtc tagatcaaca agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt
      721 cagtcgacat ggctacaggg agtgcccagg gcaacttcac tggacatacc aagaagacaa
      781 atggcaataa tggcaccaat ggcgcactcg tccaaagccc ttctaatcag agtgcccttg
      841 gagcaggggg agcgaacagt aatggaagtg cggccagagt gtggggtgta gccacaggct
      901 ccagctctgg cctggctcac tgctctgtca gtggtgggga tggaaaaatg gacactatga
      961 ttggagatgg gagaagtcag aattgctggg gtgcttccaa ctccaatgct ggcattaatc
     1021 ttaaccttaa tcctaatgcc aacccagctg cctggcctgt acttggacat gaaggaaccg
     1081 tggcgacagg caacccttcc agtatttgca gtccagtcag tgccataggt caaaatatgg
     1141 gcaaccagaa cgggaaccca acaggcactt taggtgcttg gggaaacttg ctgccacaag
     1201 agagcacaga accacaaacg tccacttctc agaatgtgtc tttcagcgca caacctcaga
     1261 accttaacac tgatggacca aataacacta accccatgaa ctcttcaccc aaccctatca
     1321 atgcaatgca gacaaatgga ctgccaaact ggggcatggc tgttggtatg ggggccatca
     1381 tcccgcccca cctgcaaggc cttcctggtg ctaatggatc atcagtttct caagtcagtg
     1441 ggggcagtgc tgaaggaata agcaattctg tgtggggact gtccccaggt aaccctgcca
     1501 caggaaatag caattctggg ttcagtcagg ggaatggaga cactgtgaac tcagcattaa
     1561 gtgctaaaca aaatggatcc agcagtgctg tgcaaaagga aggaagtgga ggaaatgctt
     1621 gggattcagg acctcctgct ggtcctggaa tactcgcctg gggaaggggc agtggcaaca
     1681 atggcgttgg taatatccat tcaggagctt ggggccaccc cagccgaagc acctctaacg
     1741 gtgtgaatgg ggaatgggga aagcccccaa accagcattc caacagtgac atcaatggga
     1801 aaggatcaac agggtgggag agtcctagtg tcaccagcca gaaccctacc gtacagcctg
     1861 gtggtgaaca catgaactcc tgggccaaag cggcatcttc tggaactaca gcaagtgaag
     1921 gaagtagtga tggttctggc aaccacaatg aaggaagcac tgggagggaa ggaacgggag
     1981 aaggccgaag gcgagataaa gggattatag accaagggca catccagttg ccaaggaatg
     2041 atcttgaccc aagagttctg tctaatactg gttggggaca gactcctgta aagcaaaaca
     2101 ctgcctggga atttgaagaa tcccctaggt ctgaaaggaa aaatgacaat gggacagagg
     2161 cctggggttg tgcagctact caggcttcaa actcaggggg gaagaacgat gggtccatca
     2221 tgaacagtac aaatacctct tcagtatctg ggtgggtcaa cgcgccacct gccgctgtgc
     2281 cagcaaacac aggttgggga gacagcaaca acaaagcgcc aagtggcccg ggggtttggg
     2341 gggactcgat aagctctact gctgttagta ctgctgctgc tgccaagagt ggccatgctt
     2401 ggagtggggc cgcaaatcag gaggacaagt cacccacctg gggtgagcct ccaaagccca
     2461 aatcccaaca ctggggagat ggacaaagat caaatccagc ctggagtgca ggagggggag
     2521 attgggcaga ttcatcgtct gtccttggac acttggggga tgggaaaaaa aatggatctg
     2581 gatgggatgc tgacagtaat aggtcagggt ctggttggaa tgacaccacg agatctggga
     2641 acagtggctg gggcaacagc acaaatacaa aggccaatcc aggtacaaac tggggggaga
     2701 ctttaaaacc tggcccccaa cagaactggg ctagcaaacc ccaagacaac aatgtgagta
     2761 actggggagg agctgcttct gtgaaacaga caggaacagg gtggatcggg gggccggtac
     2821 cggtcaaaca gaaggacagc agtgaagcaa ctggctggga agaaccctct ccaccgtcca
     2881 ttcgccgcaa aatggaaatt gatgatggta cctcagcttg gggggaccca agcaactata
     2941 acaataaaac tgtaaacatg tgggatagaa acaacccggt catccagagc agtaccacga
     3001 ccaataccac caccaccacc accactacca cgagcaacac cacacacagg gtcgagacgc
     3061 cgcccccgca ccaggctggt actcagctga atcgatcacc gttgcttggt ccaggtagga
     3121 aagtttcatc aggctgggga gaaatgccta atgttcactc aaagactgaa aactcttggg
     3181 gagaaccatc ctccccttct accctggtgg ataatggcac agcagcatgg gggaagccac
     3241 ccagcagtgg cagcgggtgg ggagatcacc ctgccgagcc gccggtggca tttggaagag
     3301 ctggcgcacc tgttgctgcc tcagccctgt gcaaaccagc ttcaaaatct atgcaagaag
     3361 gctggggcag tggtggggat gaaatgaacc tcagtaccag ccagtgggag gatgaagaag
     3421 gggacgtgtg gaataatgct gcttcccaag aaagcacctc ctcctgcagc tcctggggga
     3481 acgcccccaa aaaaggactt caaaagggca tgaagacgtc tggcaagcag gatgaggcct
     3541 ggatcatgag ccggctgatc aaacaactca cagacatggg cttcccgaga gagccagctg
     3601 aggaggcctt gaagagtaac aatatgaatc ttgatcaggc catgagcgct ctgctggaaa
     3661 agaaggtgga cgtggacaag cgtgggctgg gagtgaccga ccataatgga atggccgcca
     3721 agcccctcgg ctgccgcccg ccaatctcca aagagtcttc cgtggaccgc cccacctttc
     3781 ttgacaagga tggcggcctc gtggaagagc ccacgccttc accgttcttg ccttccccaa
     3841 gcctgaagct ccccctttca cacagtgcac tccccagtca ggccctgggt gggattgcct
     3901 ccgggctggg catgcaaaac ttgaattctt ctagacagat accgagtggc aatctgggta
     3961 tgtttggcaa tagtggagca gcacaagcca ggaccatgca gcagccgcca cagccaccag
     4021 tgcagcctct taactcttcc cagcccagtc tccgtgctca agtgcctcag tttctatccc
     4081 ctcaggttca agcacagctt ttgcagtttg cagcaaaaaa cattggtctc aaccctgcac
     4141 tattaacctc gccaattaat cctcaacata tgacgatgtt gaaccagctc tatcagctgc
     4201 agctggcata ccaacgttta caaatccagc agcagatgtt acaggcccag cgtaatgtgt
     4261 ccggatccat gagacaacag gagcagcaag ttgcgcgcac aatcactaat ctgcagcagc
     4321 agatccagca gcaccagcgc cagctggccc aggccctgct cgtgaagcag ccaccaccgc
     4381 caccgccccc gccgcacctg tctctgcacc cctctgcagg caaatcggcc atggacagct
     4441 tcccctcgca cccacagact cccggcctac ctgacctgca gaccaaagag cagcagtctt
     4501 cacccaacac ctttgctcct taccctctcg ctggactgaa cccaaacatg aatgtcaaca
     4561 gcatggacat gaccggtggc ttgtcggtga aggacccatc ccagtcccag tcacgcctcc
     4621 cccagtggac gcaccccaac tccatggata acttgcccag tgccgcttcc cccctggagc
     4681 agaaccctag caagcatggt gctatccctg gaggtctaag cattgggcct ccaggtaagt
     4741 cctccattga tgactcctat ggccggtacg atttaatcca gaacagtgag tcaccagcca
     4801 gtcctcccgt agctgttccc catagctggt cacgtgccaa atctgacagt gataaaatct
     4861 caaatggctc tagcatcaac tggcccccag aattccatcc gggagttcca tggaaaggac
     4921 tgcagaatat tgaccctgag aatgaccctg acgtcactcc tggcagtgtc cccactgggc
     4981 ctaccatcaa caccaccatc caggatgtca accgctacct cctcaagagt ggaggtaaac
     5041 tgtcagacat caaatcgacg tggtcctctg gccctacctc ccacacgcaa gcctctctgt
     5101 ctcatgaact atggaaggtg cccagaaaca gtactgcacc cacgaggcca cctccagggt
     5161 taaccaatcc caagccctcc tccacctggg gtgccagccc cctcggctgg accagctcct
     5221 actcctcggg ttctgcctgg agcaccgaca cctcaggaag aaccagcagc tggctcgttc
     5281 ttcgaaacct cactccccag attgatggtt ctacactgcg gacattgtgt ttgcaacatg
     5341 ggcctcttat cacattccac ctgaatctga ctcaaggcaa tgctgtggtc cggtacagct
     5401 ccaaggagga ggctgccaag gcccagaagt ctctgcacat gtgcgtcctg ggaaacacta
     5461 ccatcctggc cgagttcgct ggtgaagaag aagtgaatcg cttcttagcc caaggccagg
     5521 cgctgccacc cacttccagc tggcagtcca gcagcgcgtc cagccagccg cggctcagcg
     5581 cagcgggcag ctcccatggc ctggtacgca gcgacgctgg ccactggaac gccccgtgcc
     5641 tgggtggcaa ggggagcagt gagctgctgt ggggcggggt gccccagtac tccagcagcc
     5701 tgtggggccc gcccagcgcc gacgacagca gggtgatagg cagccccacg ccgctaacca
     5761 ccctgctgcc tggggacctg ctcagcgggg agtccctgta ggcggccgca ctcgagatat
     5821 ctagacccag ctttcttgta caaagtggtt gatcgcgtgc atgcgacgtc atagctctct
     5881 ccctatagtg agtcgtatta taagctaggc actggccgtc gttttacaac gtcgtgactg
     5941 ggaaaactgc tagcttggga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     6001 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     6061 atgtgttaaa ctagctgcat atgcttgctg cttgagagtt ttgcttactg agtatgattt
     6121 atgaaaatat tatacacagg agctagtgat tctaattgtt tgtgtatttt agattcacag
     6181 tcccaaggct catttcaggc ccctcagtcc tcacagtctg ttcatgatca taatcagcca
     6241 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     6301 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     6361 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6421 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgatc ctgcattaat
     6481 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctggcgtaat agcgaagagg
     6541 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     6601 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6661 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6721 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6781 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6841 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6901 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6961 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaatattt aacgcgaatt
     7021 ttaacaaaat attaacgttt acaatttcgc ctgatgcggt attttctcct tacgcatctg
     7081 tgcggtattt cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg
     7141 aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     7201 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7261 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7321 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7381 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7441 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7501 ttggaggcct aggcttttgc aaaaagcttg attcttctga cacaacagtc tcgaacttaa
     7561 ggctagagcc accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     7621 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     7681 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     7741 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     7801 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     7861 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     7921 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     7981 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     8041 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     8101 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     8161 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     8221 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     8281 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     8341 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     8401 gcgacgccca acctgccatc acgatggccg caataaaata tctttatttt cattacatct
     8461 gtgtgttggt tttttgtgtg aatcgatagc gataaggatc cgcgtatggt gcactctcag
     8521 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     8581 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     8641 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     8701 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     8761 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8821 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8881 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8941 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9001 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9061 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9121 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9181 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9241 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9301 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9361 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9421 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9481 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9541 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9601 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9661 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9721 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9781 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9841 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9901 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9961 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10021 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
    10081 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10141 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10201 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10261 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
    10321 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10381 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10441 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10501 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10561 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10621 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10681 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10741 atgcagagct tgcaattcgc gcgtttttca atattattga agcatttatc agggttattg
    10801 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10861 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10921 ctataaaaat aggcgtagta cgaggccctt tcact
//
