LOCUS       sequence-9398-ex        8894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 8894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pDESTmycMOV10
FEATURES             Location/Qualifiers
     source          1..8894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        319..522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     473..493
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     519..543
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             639..668
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    678..702
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3351..3362
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(3764..3788)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3820..3839)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3821..3839)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3850..3867)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3850..3866)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3859..3881)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    4271..4405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4308..4327)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4362..4381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4531..4986
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4618..4637)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4828..4849
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5105..5462
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5105..5296
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5128..5148)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5313..5448
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5513..6307
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5567..5586)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6177..6196
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6371..6419
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6460..6479)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6579..6601
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6639..6657)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6725..6829
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6830..6898,6899..7690)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7048..7067)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7861..8449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8350..8369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8603..8620
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cattagatgc atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
       61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      601 agcctccgga ctctagccta ggccgcggta ccaccatgga acaaaaactt atttctgaag
      661 aagatctgtc tagatcaaca agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt
      721 cagtcgacat gcccagtaag ttcagctgcc ggcagctccg ggaggcgggc cagtgtttcg
      781 agagtttcct ggtcgttcgg ggactggaca tggagacaga tcgcgagcgg ctgcggacca
      841 tttataaccg cgacttcaag atcagctttg ggacccccgc ccctggcttc tcctccatgc
      901 tgtatggaat gaagattgca aatctggcct acgtcaccaa gactcgggtc aggttcttca
      961 gactcgaccg ctgggccgac gtgcggttcc cagaaaagag gagaatgaag ctggggtcag
     1021 atatcagcaa acaccacaag tcactgctag ccaagatctt ttatgacagg gctgagtatc
     1081 ttcatgggaa acatggtgtg gatgtggaag tccaggggcc ccatgaagcc cgagatgggc
     1141 agctccttat ccgcctggat ttgaaccgca aagaggtgct gaccctgagg cttcggaatg
     1201 gcggaaccca gtctgttacc ctcactcacc tcttcccact ctgccggaca ccccagtttg
     1261 ctttctacaa tgaagaccag gagttgccct gtccactggg ccccggtgaa tgctatgaac
     1321 tccatgtcca ttgtaagacc agctttgtgg gctacttccc agccacagtg ctctgggagc
     1381 tgctgggacc tggggagtcg ggttcagaag gagccggcac attctacatt gcccgcttct
     1441 tggctgccgt cgcccacagc cccctggctg cacagctgaa gcccatgact cccttcaagc
     1501 ggacccggat caccggaaac cctgtggtga ccaatcggat agaggaagga gagagacctg
     1561 accgcgctaa gggctatgac ctggagttaa gtatggcgct ggggacatac tacccacctc
     1621 cccgcctcag gcagctgctc cccatgcttc ttcagggaac aagtatcttc actgccccta
     1681 aggagatcgc agagatcaag gcccagctgg agacagccct gaagtggagg aactatgagg
     1741 tgaagctgcg gctgctgctg cacctggagg aactgcagat ggagcatgat atccggcact
     1801 atgacctgga gtcggtgccc atgacctggg accctgtgga ccagaacccc aggctgctca
     1861 cgctggaggt tcctggagtg actgagagcc gcccctcagt gctacggggc gaccacctgt
     1921 ttgccctttt gtcctcggag acacaccagg aggaccccat cacatataag ggctttgtgc
     1981 acaaggtgga attggaccgt gtcaagctga gcttttccat gagcctcctg agccgctttg
     2041 tggatgggct gaccttcaag gtgaacttta ccttcaaccg ccagccgctg cgagtccagc
     2101 accgtgccct ggagctgaca gggcgctggc tgctgtggcc catgctcttt cctgtggcac
     2161 ctcgggacgt cccgctgctg ccctcagatg tgaaactcaa gctgtacgac cggagtctgg
     2221 agtcaaaccc agagcagctg caggccatga ggcacattgt tacgggcacc acccgtccag
     2281 ccccctacat catctttggg cctccaggca ccggcaagac tgtcacgtta gtggaggcaa
     2341 ttaagcaggt ggtgaagcac ttgcccaaag cccacatctt ggcctgcgct ccatccaact
     2401 caggggctga cctactctgt caaaggctcc gggtccacct tcctagctcc atctaccgcc
     2461 tcctggcccc cagcagggac atccgcatgg tacctgagga catcaagccc tgctgcaact
     2521 gggacgcaaa gaagggggag tatgtatttc ccgccaagaa gaagctgcag gaataccggg
     2581 tcttaattac caccctcatc actgccggca ggttggtctc ggcccagttt cccattgatc
     2641 acttcacaca catcttcatc gatgaggctg gccactgcat ggagcctgag agtctggtag
     2701 ctatagcagg gctgatggaa gtaaaggaaa caggtgatcc aggagggcag ctggtgctgg
     2761 caggagaccc tcggcagctg gggcctgtgc tgcgttcccc actgacccag aagcatggac
     2821 tgggatactc actgctggag cggctgctca cctacaactc cctgtacaag aagggccctg
     2881 atggctatga cccccagttc ataaccaagc tgctccgcaa ctacaggtct catcccacca
     2941 tcctggacat tcctaaccag ctctattatg aaggggagct gcaggcctgt gctgatgtcg
     3001 tggatcgaga acgcttctgc cgctgggcgg gcctacctcg acagggcttt cccatcatct
     3061 ttcacggcgt aatgggcaaa gatgagcgtg aaggcaacag cccatccttc ttcaaccctg
     3121 aagaggctgc cacagtgact tcctacctga agctgctcct ggccccctcc tccaagaagg
     3181 gcaaagctcg cctgagccct cgaagtgtgg gcgtcatctc cccgtaccgg aaacaggtgg
     3241 agaaaatccg ttactgcatc accaaacttg acagggagct tcgaggactg gatgacatca
     3301 aggacttgaa ggtgggttca gtagaagaat tccaaggcca agaacgaagc gtcatcctca
     3361 tctccaccgt gcgaagcagc cagagctttg tgcagctgga tctggacttt aatctgggtt
     3421 tccttaagaa ccccaagagg ttcaatgtag ctgtgacccg ggccaaggcc ctgctcatca
     3481 tcgtggggaa cccccttctc ctgggccatg accctgactg gaaagtattc ctggagttct
     3541 gtaaagaaaa cggagggtat accgggtgtc ccttccctgc caaactggac ctgcaacagg
     3601 gacagaattt actgcaaggt ctgagcaagc tcagcccctc tacctcaggg ccccacagcc
     3661 atgactacct cccccaggag cgggagggtg aagggggcct gtctctgcaa gtggagccag
     3721 agtggaggaa tgagctctga gcggccgcac tcgagatatc tagacccagc tttcttgtac
     3781 aaagtggttg atcgcgtgca tgcgacgtca tagctctctc cctatagtga gtcgtattat
     3841 aagctaggca ctggccgtcg ttttacaacg tcgtgactgg gaaaactgct agcttgggat
     3901 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     3961 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tagctgcata
     4021 tgcttgctgc ttgagagttt tgcttactga gtatgattta tgaaaatatt atacacagga
     4081 gctagtgatt ctaattgttt gtgtatttta gattcacagt cccaaggctc atttcaggcc
     4141 cctcagtcct cacagtctgt tcatgatcat aatcagccat accacatttg tagaggtttt
     4201 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     4261 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4321 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4381 caatgtatct tatcatgtct ggatcgatcc tgcattaatg aatcggccaa cgcgcgggga
     4441 gaggcggttt gcgtattggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4501 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     4561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4741 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4861 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4921 taaaaaatga gctgatttaa caaatattta acgcgaattt taacaaaata ttaacgttta
     4981 caatttcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     5041 cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta
     5101 ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5161 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5221 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5281 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5341 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5401 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5461 aaaagcttga ttcttctgac acaacagtct cgaacttaag gctagagcca ccatgattga
     5521 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5581 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5641 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5701 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5761 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5821 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5881 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5941 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6001 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     6061 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6121 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6181 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6241 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6301 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     6361 cgatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     6421 atcgatagcg ataaggatcc gcgtatggtg cactctcagt acaatctgct ctgatgccgc
     6481 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     6541 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     6601 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     6661 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     6721 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6781 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6841 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6901 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6961 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7021 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7081 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7141 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7201 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7261 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7321 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7381 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7441 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7501 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7561 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7621 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7681 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7741 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7801 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7861 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7921 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7981 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     8041 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8101 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8161 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8221 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     8281 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8341 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8401 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8461 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8521 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8581 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8641 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagagctt gcaattcgcg
     8701 cgtttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8761 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8821 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtagtac
     8881 gaggcccttt cact
//
