LOCUS       sequence-93989-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for baculovirus expression of CAMSAP1 in Sf9 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pFasBac+GFP-CAMSAP1
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..41)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(63..80)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(147..238)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(205..222)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(590..595,596..612,613..618)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttgctcactg gatccatggc gccctgaaaa tacaggtttt cggtcgttgg gatatcgtaa
       61 tcgtgatggt gatggtgatg gtagtacgac atggtttcgg accgagatcc gcgcccgatg
      121 gtgggacggt atgaataatc cggaatattt ataggttttt ttattacaaa actgttacga
      181 aaacagtaaa atacttattt atttgcgaga tggttatcat tttaattatc tccatgatct
      241 attaatattc cggagtatac gtagccaacc actagaacta tagctagagt cctgggcgaa
      301 caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc acttcatccg gggtcagcac
      361 caccggcaag cgccgcgacg gccgaggtct tccgatctcc tgaagccagg gcagatccgt
      421 gcacagcacc ttgccgtaga agaacagcaa ggccgccaat gcctgacgat gcgtggagac
      481 cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa
      541 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt tgacataagc
      601 ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
      661 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      721 tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
      781 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcg
//
