LOCUS       sequence-93990-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Pfu DNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Goldman S, Kim R, Hung LW, Jancarik J, Kim SH
  TITLE     Purification, crystallization and preliminary X-ray crystallographic
            analysis of Pyrococcus furiosus DNA polymerase.
  JOURNAL   Acta Crystallogr D Biol Crystallogr. 1998 Sep 1. 54(Pt 5):986-8.
  PUBMED    9757114
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr D Biol Crystallogr. 1998 Sep 1. 54(Pt 5):986-8."
COMMENT     Sequence Label: pET16B.Pfu
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..57
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             70..81
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 actttaagaa ggagatatac catgggccat catcatcatc atcatcatca tcatcacagc
       61 agcggccata tcgaaggtcg tcatatgatt ttagatgtgg attacataac tgaagaagga
      121 aaacctgtta ttaggctatt caaaaaagag aacggaaaat ttaagataga gcatgataga
      181 acttttagac catacattta cgctcttctc agggatgatt caaagattga agaagttaag
      241 aaaataacgg gggaaaggca tggaaagatt gtgagaattg ttgatgtaga gaaggttgag
      301 aaaaagtttc tcggcaagcc tattaccgtg tggaaacttt atttggaaca tccccaagat
      361 gttcccacta ttagagaaaa agttagagaa catccagcag ttgtggacat cttcgaatac
      421 gatattccat ttgcaaagag atacctcatc gacaaaggcc taataccaat ggagggggaa
      481 gaagagctaa agattcttgc cttcgatata gaaaccctct atcacgaagg agaagagttt
      541 ggaaaaggcc caattataat gattagttat gcagatgaaa atgaagcaaa ggtgattact
      601 tggaaaaaca tagatcttcc atacgttgag gttgtatcaa gcgagagaga gatgataaag
      661 agatttctca ggattatcag ggagaaggat cctgacatta tagttactta taatggagac
      721 tcattcgact tcccacattt agcgaaaagg gcagaaaaac ttggg
//
