LOCUS       sequence-93996-e        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated 
            transformation). There is a 68bp MCS (multiple cloning site) which 
            contains 12 unique restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Yang F, Naqvi NI
  TITLE     Sulfonylurea resistance reconstitution as a novel strategy for 
            ILV2-specific integration in Magnaporthe oryzae.
  JOURNAL   Fungal Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005.
  PUBMED    24769367
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005."
COMMENT     Sequence Label: pFGL815N
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(54..73)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     214..236
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      472..666
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
       61 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
      121 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
      181 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
      241 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
      301 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
      361 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
      421 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
      481 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
      541 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
      601 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
      661 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
      721 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
      781 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
      841 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
      901 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
      961 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
     1021 agcacgtccg ggttcttgcc tt
//
