LOCUS       sequence-94-expo        8838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 8838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_16
FEATURES             Location/Qualifiers
     source          1..8838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1755..1775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1865..1887)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6278..6300)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6400..6419
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6497..6515)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6583..6687
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6688..6756,6757..7548)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6906..6925)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7719..8307
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8208..8227
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8461..8478
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8595..8616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8631..8636,8637..8654,8655..8661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8669..8685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8674..8696
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8693..8709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8693..8709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggc cttggtcgag tatgagaaac aacattaaca atgttttggg
       61 agaaacatgt agttttgtac gtccggtcca gtgagagttt tcaacttatt ttggaagaaa
      121 tttttttttg acaaaatttt gcaatttccc acgcagaaat acggtatgcc ccgtatctcg
      181 ccacgaacat aatattttaa attgacggaa aggtgtgcgc ctttaagaag cacagtaacc
      241 attttccggt tttttttttg aaattgcctt tgatttttac ttttttctcc caaaaacgag
      301 aaatttaata taatccatca aaatctttgt gaacgagaga aatatagtga aaaactcaaa
      361 gaaaattgcg aaatggatta ctgtactcca taaaggcgca catcttttcg aaaaatcgtg
      421 aaaaattgca tctgaccaac agttttgcgt ggaatcttcc tagattcaaa tttcctatga
      481 gtcataaaac tatcatttta tcggacaata actacacgtg taccttgttt ccatatgttc
      541 taccaatata atttgtgttc atatgtggcc aggtacggga aattagaaca cggactgatt
      601 taggaaggtt caaattcgag aacaatggac gacttgctgc acgaaaacgt ggattattct
      661 gaaaccgcct atgtgatcac tatcgattta gcacttcggt tttctttttc cgttttcctt
      721 tatattccgt attgtatgcc gacaataacg ctttttcttt gtataaacat ttccagaagc
      781 agagcaaatt tgtttattcc agaattgact gaatattgaa ataatgttta cagacatact
      841 gtagattttt ttctattcaa acatgaatac agtttttcgt atttttaatc ttcagataag
      901 ttttgtgtgt gtttttgaga ataatattta aaaaagataa gcaaatatca gaactacggt
      961 aagcgaaggg gtacggtcgt caattgcgga cttccgagct gtgcacggcg ccaaaattta
     1021 ttttgagttt taaaatgatt ttctttgttt ttttttcttt ttttgacacg ataagaacat
     1081 ttttctgtat ttctaatatt ttaaatgaga tcgtaataag tttatttagc tccatcacat
     1141 tattttcgga atattcatcg cttgaaaacg gcgtaaaata ggttccaagt aaatcattgc
     1201 aaacaaatct ccgtttatcg ttctaacaaa gtttttcgtt cactttctta tgtaaatcgt
     1261 tttccccact tcttcttcac gggaatgatc aattgaaagg ccataaattg gttgttttct
     1321 ccacatttcg caaccaacat gcagctgtcg gataggcaat aaaataatgt aatcaatgag
     1381 ttttccgatt tatcctaaca gttctttttc tagtgttcct attcgaaaaa agtactgtta
     1441 tccttctttt tggaccgccc atctcaggtg tcaagacaat cgcaacacac tcagaaaatg
     1501 ggcggagtat gccacccggc cttcattcgt cgctccttgt cttctccatt ttcgtttttt
     1561 tcctgttctc cgttcttttt ttgtactttt gtgataatgc tgaagtgttc ttttttttcg
     1621 cttaaccgca tctcttgatt taattcatat tttctttcag attcatatac aataacacca
     1681 agagctgctc taacaactaa taataacaga atctagagga tccccgggta ccgagctcag
     1741 aaaaaatgac tgctccaaag aagaagcgta aggtaccggt gggtgaagac cagaaacagc
     1801 acctcgaact gagccgcgat attgcccagc gtttcaacgc gctgtatggc gagatcgatc
     1861 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     1921 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     1981 cccaacagtt gcgaaggtaa gtttaaacag atccatacta actaacttgt tctgacataa
     2041 ttttcagctt gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
     2101 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc
     2161 agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc
     2221 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg
     2281 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc
     2341 atctgtggtg caacgggcgc tgggtcggtt acggccaggt aagtttaatt aagttgatac
     2401 taactaacaa gatctgatta attttcagga cagtcgtttg ccgtctgaat ttgacctgag
     2461 cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg
     2521 cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt
     2581 gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga
     2641 tttctcccgc gctgtactgg aggctgaagt ccaggtaagt ttaaacagga tcttactaac
     2701 taacatgcta acactgaatt ttcagatgtg cggcgagttg cgtgactacc tacgggtaac
     2761 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga
     2821 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa
     2881 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac
     2941 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat
     3001 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca
     3061 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatgtaag
     3121 tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagatcc tgctgatgaa
     3181 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
     3241 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
     3301 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt
     3361 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
     3421 tggtaagttt aaacagttga atactaacta acggagatct ttgaaatttt cagaatcagg
     3481 ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc
     3541 ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta
     3601 cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg
     3661 gctttcgcta cctggagaga cgcgcccgct gattctttgc gaggtaagtt taaacagaac
     3721 tctactaact aacacattag atcctaattt tcagtacgct cacgcgatgg gcaacagtct
     3781 tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt
     3841 cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc
     3901 ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct
     3961 ggtctttgcc gaccgcacgc cgcatccagc gtaagtttaa acaataacct actaactaac
     4021 gtagataatt taaattttca ggctgacgga agcaaaacac cagcagcagt ttttccagtt
     4081 ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa
     4141 cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc
     4201 tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga
     4261 gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc
     4321 agaagccggg cacatcagcg catggcagca gtggaggtaa gtttaaacaa gatcctacta
     4381 actaactcta cattgatgaa ttttcagact ggcggaaaac ctcagtgtga cgctccccgc
     4441 cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg
     4501 taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga
     4561 taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga
     4621 cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcggta agtttaaaca
     4681 aagttgtact aactaacgaa gatcttgata attttcagaa cgctggaagg cggcgggcca
     4741 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct
     4801 gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac
     4861 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga
     4921 tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt
     4981 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt
     5041 tgaccgctgg gatctgccat tgtcagacat gtagtaagtt taaacttgat agtactaact
     5101 aacatgtttc atttaaattt tcagtacccc gtacgtcttc ccgagcgaaa acggtctgcg
     5161 ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa
     5221 catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc
     5281 ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc
     5341 ctggagcccg tcagtatcgg cggaattcca actgagcgcc ggtcgctacc attaccaact
     5401 tgtctggtgt caaaaataat aggggccgct gtcatcagag taagtttaaa ctgagttcta
     5461 ctaactaacg agtaatattt aaattttcag catctcgcgc ccgtgcctct gacttctaag
     5521 tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt
     5581 tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc
     5641 taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa
     5701 attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg
     5761 tacacttctt atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc
     5821 acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg
     5881 cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt
     5941 ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg
     6001 ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag
     6061 tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa
     6121 ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat
     6181 gacagaatac attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc
     6241 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     6301 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     6361 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     6421 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     6481 tcaggcggcc ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6541 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6601 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6661 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6721 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6781 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6841 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6901 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6961 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7021 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7081 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7141 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7201 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7261 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7321 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7381 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7441 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7501 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7561 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7621 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7681 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7741 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7801 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7861 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7921 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7981 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8041 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8101 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8161 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8221 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8281 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8341 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8401 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8461 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8521 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     8581 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8641 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8701 agctatgacc atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt
     8761 ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta
     8821 acaacttgga aatgaaat
//
