LOCUS       sequence-9400-ex        7817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pDESTmycPABPC4
FEATURES             Location/Qualifiers
     source          1..7817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        319..522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     473..493
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     519..543
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             639..668
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    678..702
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2687..2711)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2743..2762)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2744..2762)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2773..2790)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2773..2789)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2782..2804)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    3194..3328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3231..3250)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3285..3304
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3454..3909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3541..3560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3751..3772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4028..4385
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4028..4219
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4051..4071)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4236..4371
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4436..5230
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4490..4509)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5100..5119
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5294..5342
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5383..5402)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5502..5524
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5562..5580)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5648..5752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5753..5821,5822..6613)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5971..5990)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6784..7372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7273..7292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7526..7543
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cattagatgc atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
       61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      601 agcctccgga ctctagccta ggccgcggta ccaccatgga acaaaaactt atttctgaag
      661 aagatctgtc tagatcaaca agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt
      721 cagtcgacat gaacgctgcg gccagcagct accccatggc ctccctgtac gtgggcgacc
      781 tgcattcgga cgtcaccgag gccatgctgt acgaaaagtt cagccccgcg gggcctgtgc
      841 tgtccatccg ggtctgccgc gatatgatca cccgccgctc cctgggctat gcctacgtca
      901 acttccagca gccggccgac gctgagcggg ctttggacac catgaacttt gatgtgatta
      961 agggaaagcc aatccgcatc atgtggtctc agagggatcc ctctttgaga aaatctggtg
     1021 tgggaaacgt cttcatcaag aacctggaca aatctataga taacaaggca ctttatgata
     1081 ctttttctgc ttttggaaac atactgtcct gcaaggtggt gtgtgatgag aacggctcta
     1141 agggttatgc ctttgtccac ttcgagaccc aagaggctgc cgacaaggcc atcgagaaga
     1201 tgaatggcat gctcctcaat gaccgcaaag tatttgtggg cagattcaag tctcgcaaag
     1261 agcgggaagc tgagcttgga gccaaagcca aggaattcac caatgtttat atcaaaaact
     1321 ttggggaaga ggtggatgat gagagtctga aagagctatt cagtcagttt ggtaagaccc
     1381 taagtgtcaa ggtgatgaga gatcccaatg ggaaatccaa aggctttggc tttgtgagtt
     1441 acgaaaaaca cgaggatgcc aataaggctg tggaagagat gaatggaaaa gaaataagtg
     1501 gtaaaatcat atttgtaggc cgtgcacaaa agaaagtaga acggcaggca gagttaaaac
     1561 ggaaatttga acagttgaaa caggagagaa ttagtcgata tcagggggtg aatctctaca
     1621 ttaagaactt ggatgacact attgatgatg agaaattaag gaaagaattt tctccttttg
     1681 gatcaattac cagtgctaag gtaatgctgg aggatggaag aagcaaaggg tttggcttcg
     1741 tctgcttctc atctcctgaa gaagcaacca aagcagtcac tgagatgaat ggacgcattg
     1801 tgggctccaa gccactatat gttgccctgg cccagaggaa ggaagagaga aaggctcacc
     1861 tgaccaacca gtatatgcaa cgagtggctg gaatgagagc acttcctgcc aatgccatct
     1921 taaatcagtt ccagcctgca gcgggtggct actttgtgcc agcagtccca caggctcagg
     1981 gaaggcctcc atattataca cctaaccagt tagcacagat gaggcctaat ccacgctggc
     2041 agcaaggtgg gagacctcaa ggcttccaag gaatgccaag tgctatacgc cagtctgggc
     2101 ctcgtccaac tcttcgccat ctggctccaa ctgggtctga gtgcccggac cgcttggcta
     2161 tggactttgg tggggctggt gccgcccagc aagggctgac tgacagctgc cagtctggag
     2221 gcgttcccac agctgtgcag aacttagcgc cacgcgctgc tgttgctgct gctgctcccc
     2281 gggctgttgc cccctacaaa tacgcctcca gtgtccgcag ccctcatcct gccatacagc
     2341 ctctgcaggc accccagcct gcggtccatg tgcaggggca ggagccactg actgcctcca
     2401 tgctggctgc agcacccccc caggaacaga agcagatgct gggagaacgc ttgttcccac
     2461 tcatccaaac aatgcattca aatctggctg ggaagatcac gggaatgctg ctggagatag
     2521 acaactctga gctgctgcac atgttagagt cccccgagtc tctccgctcc aaggtggatg
     2581 aagctgtagc agttctacag gctcatcatg ccaagaaaga agctgcccag aaggtgggcg
     2641 ctgttgctgc tgctacctct taggcggccg cactcgagat atctagaccc agctttcttg
     2701 tacaaagtgg ttgatcgcgt gcatgcgacg tcatagctct ctccctatag tgagtcgtat
     2761 tataagctag gcactggccg tcgttttaca acgtcgtgac tgggaaaact gctagcttgg
     2821 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     2881 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactagctgc
     2941 atatgcttgc tgcttgagag ttttgcttac tgagtatgat ttatgaaaat attatacaca
     3001 ggagctagtg attctaattg tttgtgtatt ttagattcac agtcccaagg ctcatttcag
     3061 gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat ttgtagaggt
     3121 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     3181 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3241 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3301 catcaatgta tcttatcatg tctggatcga tcctgcatta atgaatcggc caacgcgcgg
     3361 ggagaggcgg tttgcgtatt ggctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3421 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     3481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3661 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3721 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3781 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3841 ggttaaaaaa tgagctgatt taacaaatat ttaacgcgaa ttttaacaaa atattaacgt
     3901 ttacaatttc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3961 atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag
     4021 gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4081 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4141 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4201 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4261 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4321 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4381 gcaaaaagct tgattcttct gacacaacag tctcgaactt aaggctagag ccaccatgat
     4441 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     4501 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     4561 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     4621 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4681 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4741 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4801 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4861 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4921 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4981 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     5041 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     5101 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5161 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5221 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     5281 tcacgatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     5341 tgaatcgata gcgataagga tccgcgtatg gtgcactctc agtacaatct gctctgatgc
     5401 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     5461 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5521 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     5581 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5641 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5701 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5761 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5821 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5881 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5941 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6001 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6061 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6121 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6181 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6241 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6301 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6361 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6421 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6481 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6541 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6601 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6661 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6721 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6781 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6841 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6901 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     6961 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7021 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7081 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7141 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     7201 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7261 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7321 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7381 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7441 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7501 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7561 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagag cttgcaattc
     7621 gcgcgttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7681 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7741 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtag
     7801 tacgaggccc tttcact
//
