LOCUS       sequence-94022-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His-tagged EGFP-mOrange fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Tome JM, Ozer A, Pagano JM, Gheba D, Schroth GP, Lis JT
  TITLE     Comprehensive analysis of RNA-protein interactions by 
            high-throughput sequencing-RNA affinity profiling.
  JOURNAL   Nat Methods. 2014 Jun;11(6):683-8. doi: 10.1038/nmeth.2970. Epub 
            2014 May 8.
  PUBMED    24809628
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2970"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "683-8"
COMMENT     Sequence Label: EGFP-mOrange pHis-//
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     145..166
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     679..698
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
       61 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
      121 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      181 cactctcggc atggacgagc tgtacaaggg tggaggcgga tcaggtggag gcggatcagg
      241 tggaggcgga tcaggaggtg gaggttcggt gagcaagggc gaggagaata acatggccat
      301 catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt
      361 cgagatcgag ggcgagggcg agggccgccc ctacgagggc tttcagaccg ctaagctgaa
      421 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcaccta
      481 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttca agctgtcctt
      541 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      601 gacccaggac tcctccctcc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
      661 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
      721 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagatga ggctgaagct
      781 gaaggac
//
