LOCUS       sequence-94027-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses constitutively active human PKCi with a deletion on the 
            PB1 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Forteza R, Wald FA, Mashukova A, Kozhekbaeva Z, Salas PJ
  TITLE     Par-complex aPKC and Par3 cross-talk with innate immunity NF-kappaB 
            pathway in epithelial cells.
  JOURNAL   Biol Open. 2013 Oct 8;2(11):1264-9. doi: 10.1242/bio.20135918. 
            eCollection 2013.
  PUBMED    24244864
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/bio.20135918"; journalName: "Biol Open"; date: "2013-10-8- 
            8"; volume: "2"; issue: "11"; pages: "1264-9"
COMMENT     Sequence Label: pLenti6.3/V5-DEST-PKCi-A120E-Delta-PB1
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..89)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    142..166
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgat cattactaac cggtacgcgt agaatcgaga
       61 ccgaggagag ggttagggat aggcttacct tcgaaccgcg ggccctctag actcgagcgg
      121 ccgccactgt gctggatatc aaccactttg tacaagaaag ctgggtcggc gcgcccaccc
      181 ttgacacatt cttctgcaga catcaaaaga ggattgatat actcaaaacc ttcaaattca
      241 gactgatcaa tcttcctcac aatgtcatcg tcatctggag tgagctggac aggttcatta
      301 gtaaactgag aatcaaagtt gtccaaacca aattccccag aaatatttgg tttaaaggga
      361 ggtaccacct gtttttgctc catcatatcc caatcaacat ttcggaagaa cgggtgtccc
      421 tgaatatcag caaatcctgt ttgaggatga caacccaatc gttccttagg gtccttatta
      481 agaaaactct tcagaacact tgcagctttt acagacagag aacgtggtat gcgaatttgt
      541 ttttccaaaa taacttggaa gagataatcc tctgtgttct ggtcagggtt atcggagctc
      601 ccaacaatat caaatggaga ccttcctgcc atcatctcaa acatgagcac tccaagagcc
      661 caccagtcaa cactgaaacc ataatcttct cctcttaaaa tttcaggagc aatgtaatta
      721 ggagtaccac agaaagtgct ggttgtatct cctggccgta atccttcctt acacatgcc
//
