LOCUS       sequence-94034-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of mCherry under the GFAP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Perea G, Yang A, Boyden ES, Sur M
  TITLE     Optogenetic astrocyte activation modulates response selectivity of 
            visual cortex neurons in vivo.
  JOURNAL   Nat Commun. 2014;5:3262. doi: 10.1038/ncomms4262.
  PUBMED    24500276
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014"; volume: "5:3262. doi"; pages: " 
            10.1038/ncomms4262"
COMMENT     Sequence Label: pAAV-GFAP104-mCherry
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..773)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(341..360)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     610..628
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      770..779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt tgtacagaat
       61 tcttacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac
      121 tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg
      181 taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg
      241 tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc
      301 tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg cattacgggg
      361 ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc
      421 agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc
      481 cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc
      541 acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcagg
      601 gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg
      661 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
      721 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac catggtggcg
      781 gatccccgcg agcagcggag gtgatgcgtc tcctctccaa cctgctctgg ctctgctcgc
      841 tcctgggatg cgagggcttt atgaaggagt gggctagact ggcgatgccc gggtgcccct
      901 ggcaacaccc cctggcatag cctgaggggg tggggttggg ccgcagccca gctatgggga
      961 gagctctccc ctcacccatt tgtgtccttg gacttctggg aagcagacag c
//
