LOCUS       sequence-94038-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM
  TITLE     Localization of ASH1 mRNA particles in living yeast.
  JOURNAL   Mol Cell. 1998 Oct . 2(4):437-45.
  PUBMED    9809065
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            1998 Oct . 2(4):437-45."
COMMENT     Sequence Label: pSL-MS2-6X
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(248..266)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(287..305)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(357..375)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(396..414)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(466..484)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(505..523)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     primer_bind     complement(619..635)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(619..635)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(632..654)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    643..659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(667..673,674..691,692..697))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    712..733
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aggtgcacgg cccacgtggc cactagtact tctcgagctc tgtacatgtc cgcggtcgcg
       61 acgtacgcgt atcgatggcg ccagctgcag gcggccgcca tatgcatcct aggcctatta
      121 atattccgga gtatacgtag ccggctaacg ttaacaaccg gtacctctag aactatagct
      181 agcatgcgca aatttaaagc gctgatatcg atcgcgcgca gatctaatga acccgggaat
      241 actgcagaca tgggtgatcc tcatgttttc tggagtcgac ctgcagacat gggtgatcct
      301 catgttttct aggcaattag gtaccttagg atctaatgaa cccgggaata ctgcagacat
      361 gggtgatcct catgttttct agagtcgacc tgcagacatg ggtgatcctc atgttttcta
      421 ggcaattagg taccttagga tctaatgaac ccgggaatac tgcagacatg ggtgatcctc
      481 atgttttcta gagtcgacct gcagacatgg gtgatcctca tgttttctag gcaattaggt
      541 accttaggat ccatatatag ggcccgggtt ataattacct caggtcgacg tcccatggcc
      601 attcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      661 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      721 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      781 ccagctgcat taatgaatcg gccaacgcgc gggg
//
