LOCUS       sequence-94050-e       13082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable 
            for in vitro transcription (T7 promoter) for injecting mRNA into 
            oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13082)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 13082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-EGFP-ELYS-polyA
FEATURES             Location/Qualifiers
     source          1..13082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      928..937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             934..1650
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(934..936,937..939,940..1650)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(979..1000)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1240..1259)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1587..1608
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     RBS             5626..5634
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8585..8614
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8630..8647
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8670..8687)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8676..8900
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8946..9374
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9033..9052)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9243..9264
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9383..9403)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9388..9718
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9569..9704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9631..9650
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9785..10579
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9839..9858)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10449..10468
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10755..10876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10792..10811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10846..10865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10925..10941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10925..10941)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10938..10960)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10949..10965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10973..10979,10980..10997,10998..11003))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11018..11039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11156..11173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11327..11915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11407..11426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12086..12877,12878..12946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12709..12728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12947..13051)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttcga attcggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
      961 accggggtgg tgcccatcct ggtcgagctg gacggcgacg tgaacggcca caagttcagc
     1021 gtgtccggag agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1081 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1141 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1201 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1261 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1321 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1381 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1441 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1501 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1561 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1621 atcactcacg gcatggacga gctgtacaag caagacttga cagctcaagt gactagtgat
     1681 ctcctgcatt tcccagaagt gactattgaa gctcttggag aagatgagat aacattagag
     1741 tccgtgcttc gtggaaagtt tgctgcaggg aaaaatggac tagcatgctt agcttgtggt
     1801 ccacaacttg aagttgtaaa ctccttaaca ggagagcggt tatctgcata tagattcagt
     1861 ggagtaaatg aacagcctcc tgtagtcctt gcagtgaaag aattctcttg gcataagagg
     1921 actggattgt taataggatt ggaagaagca gatgggagtg ttctttgtct ttatgacctt
     1981 ggtatatcaa gagtggtcaa agcagttgtt cttcctggaa gggtaacagc tatcgagcct
     2041 ataattaacc atggaggagc cagtgcgagt acccagcatt tacatccaag tctccggtgg
     2101 ctttttggcg tggccgctgt ggtgactgat gttggacaga tccttcttat tgacctgtgt
     2161 ttggatgact tgtcctgcag tcagaatgaa gttgaggcat cagaccttga agttatcact
     2221 ggtatcccag ctgaagtacc acacatcaga gagagagtga tgagagaggg gcgccacctg
     2281 tgcttccagt tagtaagccc attgggagta gccatttcta ctctgagtta catcaacagg
     2341 acaaatcagc ttgctgtggg tttttctgat ggctacttag cactttggaa catgaaaagc
     2401 atgaaaagag aatactatac acagttggaa ggtggaaggg ttcctgtcca tgcagttgcc
     2461 tttcaagagc ctgagaatga tcctcgtaac tgctgttatt tatgggctgt tcagtccaca
     2521 caagatagtg aaggggatgt tttgagtttg catctgcttc agctggcttt tggtgataga
     2581 aaatgtttgg catcagggca aattttatat gagggattag aatactgcga agaaagatat
     2641 acactggatc tagcaggtgg cacgttcccc ttaaggggac aaactagtaa taccaaattg
     2701 ttgggatgcc agagtataga gagatttcca tctcatggag acagagaaga aagtatgaga
     2761 gaagctctgt ctcccgatac cagcgtttct gtctttacct ggcaagtgaa tatatatgga
     2821 cagggaaagc cttctgtgta tttagggcta tttgacataa atcgttggta tcatgcacaa
     2881 atgcccgatt ctttaagatc aggagaatct ctgcataatt gctcttattt tgcgttgtgg
     2941 tcattggatt cggttgtaag taggacttct ccacatcaca tcttggacat actagtacat
     3001 gagaggagtt taaaccgagg ggttcctcct tcctacccac ctccagagca attttttaac
     3061 ccaagtactt ttaattttga tgccacttgt ttgttagact ctggagttat ccatgtaact
     3121 tgtgctggat ttcagaagga gactttgaca tttttaaaga aatcaggacc aactcttaat
     3181 gaagtcattc ctgatagtta taatcgatgt cttgttgctg gtctcctctc accaagactt
     3241 attgatattc agccttccag tttaagtcaa gaagaacaat tagaagctat attgtcagca
     3301 gcaattcaga caagttcctt gggacttttg actggttaca tcagaacatg gataatagaa
     3361 gaacaaccaa attctgctgc taatctacga tttgttcttg agtggacatg gaataaagtg
     3421 gttctcacaa aagaagagtt tgataggctt tgtgtgccgc tgtttgacgg ttcgtgtcgt
     3481 tttattgacc cacagactat tcagtctatc cagcagtgcc atttactgct tagcaacctt
     3541 agtacagtct taagctgttt tgcaatggag gcccagggta tcactgagag aggactggtg
     3601 gacttgagca acaagcacat ggtcacccag cttctctgtc agtacgcaca catggttctg
     3661 tggttctgcc actcgggact tctgcccgaa ggcttagatg atgctctgca gctgtcaaga
     3721 ctacgctaca actaccctgt aattcagaac tactatacaa gtcgtcggca gaagtctgag
     3781 cgctcaccca gagggaagtg gaaccacgac tgcttgatga ttgatggatt agtctctcaa
     3841 ctaggagatg aagttgagaa gttgtggaag cgggacgaag gtggcacagg aagataccct
     3901 cctgctagca tccacgcatt acttgatata tatttattag acaacattac cgaagcaagc
     3961 aaacatgcta ttaccattta tttgctgctt gatattatgt attcctttcc aaataaaacg
     4021 gataccccca ttgaatcttt cccaactgcc tttgctattt cttggggcca agttaagcta
     4081 gttcaaggat tttggctact agatcataat gactatgaga atggtttaga ccttctgttt
     4141 cacccagtta ctgcaaagcc tgcatcgtgg caacattcaa agataattga agcttttatg
     4201 agtcagggag agcacaaaca ggctctccgg tatcttcaga caatgaagcc aacagtgtcc
     4261 agtagcaatg aagttatcct tcacctcact gttctacttt ttaatagatg catggttgag
     4321 gcctggaact tactgcgaca gaattcaaac agagtaaata tagaggaatt attaaagcac
     4381 gcttatgaag tttgtcagga gatgggctta atggaggatt tactgaagct gccatttaca
     4441 aacactgagc aggaatgctt agtgaaattt ttacagtcca gtaccagtgt tgagaatcat
     4501 gaattccttc tagttcacca tttacagcgt gccaattata tttctgcctt gaaactaaac
     4561 cagattctga agaataatct catgagtgat cgtgaccctc gattgcggga aagatcggtg
     4621 actcgaaatt ctatattaga ccagtatggg aaaatcctac ctagagtcca gagaaagtta
     4681 gctgttgagc gagctaagcc ttaccacctg tcgacatcct cagtttttca tgaagtttct
     4741 agacccaaac cgttatcggc atttccaaag aaagctataa ctggaacagt gttaacccga
     4801 tctacgttca tcagcaatgt tttatctaaa attggagagg tgtgggcaag tcatgaacct
     4861 agaaatggcg tctcactttt taacagtcct aaaacagaac agccatctcc tgtagtacac
     4921 tctttcccac acccagagct tcctgaggcg tttgttggaa ctccaatttc aaatacatcc
     4981 cagagaattt ctagattact ggatttggtt gtccatcctg taccccagcc ttctcagtgt
     5041 ttggagttta ttcaacaaag tcccacaaga tctcctttgt gtctgctgtc cagttcgtta
     5101 ccattaagtt cacagtttaa aaggccacat cagaatacct ccaggccttc agagttgctt
     5161 ttacttgaga ctcctctcat agttaagaaa gctaaatctt tggctctgtc agccacgtct
     5221 tctggatttg ccgagtttac tcctccatcc atccttaggt ctggtcttcg aacaacacct
     5281 ttagcatctc cctctttgtc acctggaaga tctctcactc cgcctttcag agttaaagaa
     5341 actaggattt cattcatgga agaaggcatg aatacacact ggactgatag agctacagat
     5401 gaccgaaata caaaagcgtt tgttagcaca tctttccata aatgtggact tccagcagaa
     5461 actgagtgga tgaagaccag tgataagaat acatattttc ctctggatgt ccctgcaaag
     5521 ggccctcaga aagtggtggc agagtcactg gctacccatt caggaaggct ggagaaactg
     5581 gatgtgagca aagaagacag cacagcttcc accaggtcag accagacctc cttagagtat
     5641 catgacgcac catcaccaga agacttggaa ggtgctgttt ttgtgtctcc caagccagca
     5701 tcttcctcca ctgaactaac tactaattca actctacaaa cagagaggga taatgataaa
     5761 gatgcgttta agtcagaagg tactccttca cccgtgaaga aacaaatagg cacgggagac
     5821 gctgcagtgg aagcattttc agaactgagt cgcttagacc ctgttgaaag agctgaagct
     5881 tcttttggtg tgtcgtcagt ctgtgaaggg gaaacctcca cttcaaactc caagacgtca
     5941 gttctggatg gaatcgtgcc tattgagagc cgaacctcca tacttacagc agaccacaaa
     6001 gagtctgtgg ccaacacggt tgcagatgtt gaaagctctg ggtccaccag ctccaagtgc
     6061 ccggttacct ctgaacgcag cctcggccaa aaactaacat taaacttaaa agaagatgaa
     6121 atagaagctc atgtaccaaa ggagaacgtt ggtttaccag aagaaagccc tcgaatttct
     6181 gctgctcctt ctgatactca cgagattcat ctaattggat gtgaaaatct tgaagttcaa
     6241 aattcagaag aggaggccaa gaatctttca tttgatgagt tgtatccctt aggggcagag
     6301 aaacttgagt ataatctcag tactattgag cagcagtttt gtgacttgcc tgatgacaaa
     6361 gactctgctg aatgtgatgc tgctgaagta gacggggaac tttttgtggc ccagagcaac
     6421 tttaccctga ttttagaagg tgaagaagga gaagctgagg caagtgactc tgcagcacct
     6481 aatatgttac caaaagcgac caaggaaaaa cctgtgagcc acagggaacc ccataatcag
     6541 gagcgcgtta cagatttgcc atctgctgtg actgctgacc aagaatccca caaggtagag
     6601 actttaccgt atgtgcctga accggttaaa gtggcaattg cagaaaatct gttggatgta
     6661 attaaagaca ccagaagtaa ggaagcaact cccgtggcag caggtgaggc tggtgatgag
     6721 gacggagcag tgatagtccc aaaggctgca cattcgtcca ggctgacaaa ctctacaccg
     6781 aagactgtta aggaaccacg tgcagagact gtaaataccc gccagagtga tgacatggtt
     6841 tcttctagaa ctctcacaag aaggcagcat gccctaagcc tgaatgtcac atcagaacaa
     6901 gagccttcag cagttgccac tcctaagaag agaactagaa aaattaaaga aactcctgag
     6961 tcttctgaaa ggacctgttc tgacctaaaa gtagcacctg agaaccaact gacagctcag
     7021 agtcctcccg ctcctaggag aggaaagaag aaggacgtta gccaaggcac actgccaagt
     7081 tctggtgctg tggagccgga acctcagggt acgccgggaa gactgaggct gagaacgcag
     7141 ccacccgagc cagcagctga agaaactcct tctagaacaa aagtcaggct ttcatctgtt
     7201 agaaagggaa cccctagaag acttaagaag tctgtagaaa atgggcaaag tacagaaatt
     7261 ctagatgatc tcaaagggag tgaggcagca agtcatgacg ggactgtcac agagctgagg
     7321 aatgccaagc tagaagatac tcagaatatg gagtataaac aagatgaaca cagtgaccag
     7381 caactgcctc taaaacgaaa gagggtcaga gagagagaag ttagtgtgtc aagtgtgaca
     7441 gaagagccaa agcttgactc atcccagttg cctcttcaga caggactcga tgtacctgcc
     7501 acccctagga aacgtggtag acccaggaag gtagttccct tagaagctga tggtggcaca
     7561 actggtaagg aacagacaag tcctcagaag aaagatgttc cggttgtccg gagatctaca
     7621 cggaacaccc cagctagaaa tgtgagtact ttagaaaaat cagttttagt gccaaataag
     7681 gaagctgctc tagtggtgac atctaagagg agacctacaa agaagtctgc agaggaaagc
     7741 tcaaaagatc catcagcggc agtctcagac ttggcgggtg gagcagccca cacagagtcc
     7801 gctgaccgaa gggacggact gcttgccgcc gctgctctca cgccatctgc ccagggcaca
     7861 aggactaggt ctagaaggac catgttgttg acggacattt ctgaacccaa aactgagcct
     7921 ttatttcctc ctccttcagt gaaggttcca aagaaaaaat caaaagctga gaacatggag
     7981 gccgcagccc agctgaaaga attggtgtca gatatatctt ctcagtttgt tgtttcccct
     8041 cctgccttga gaaccaggca gaaaagtata tccaatactt ccaagcttct aggtgaactg
     8101 gagagtgacc ctaaaccatt agagatcata gaacaaaaac caaaaagaag caggactgtg
     8161 aagacaagag caagcagaaa cacaggaaaa ggaagttctt ggtcacctcc tcctgtagaa
     8221 attaagctgg tttctccctt ggcgagtcca gtggatgaaa taaagaccgg caagccaaga
     8281 aaaactgcag aaatagcagg aaaaactctt ggaaggggca gaaagaagcc atcttctttt
     8341 ccaaagcaaa ttttacgcag gaaaatgctg taagcggccg ctcgactatg ggcaccaaag
     8401 aacctgtaaa cgttatcttt ttaaattgaa tgtgcacaaa taaaagtttg gaaaagaaaa
     8461 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     8521 aaaaaaaaaa aaaaaaaaat acgcatcata cgctgtggcg tacctcgagt ctagagggcc
     8581 cttcgaacaa aaactcatct cagaagagga tctgaatatg cataccggtc atcatcacca
     8641 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8701 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8761 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8821 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8881 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     8941 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9001 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9061 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg
     9121 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9181 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9241 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9301 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9361 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     9421 tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     9481 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9541 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9601 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     9661 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     9721 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     9781 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     9841 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     9901 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     9961 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
    10021 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
    10081 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
    10141 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
    10201 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
    10261 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
    10321 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
    10381 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
    10441 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
    10501 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
    10561 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc
    10621 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
    10681 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
    10741 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10861 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
    10921 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    10981 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    11041 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    11101 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    11161 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    11221 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    11281 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    11341 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    11401 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    11461 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    11521 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    11581 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    11641 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    11701 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    11761 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    11821 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    11881 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    11941 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    12001 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    12061 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    12121 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    12181 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    12241 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    12301 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    12361 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    12421 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    12481 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    12541 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    12601 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    12661 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    12721 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    12781 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    12841 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    12901 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    12961 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    13021 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    13081 tc
//
