LOCUS       sequence-94057-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for Chrimson-tdTomato in a floxed/forward-reading,
            Cre-dependent manner (Cre turns gene off).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pCAG-FLEX-fwd[Chrimson-tdT]
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..92
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaataggaag accaatgctt caccatcgac ccgaattgcc aagcatcacc atcgacccat
       61 aacttcgtat aatgtatgct atacgaagtt atactagcta gcgccgccac catggctgag
      121 ctgatcagca gcgccaccag atctctgttt gccgccggag gcatcaaccc ttggcctaac
      181 ccctaccacc acgaggacat gggctgtgga ggaatgacac ctacaggcga gtgcttcagc
      241 accgagtggt ggtgtgaccc ttcttacgga ctgagcgacg ccggatacgg atattgcttc
      301 gtggaggcca caggcggcta cctggtcgtg ggagtggaga agaagcaggc ttggctgcac
      361 agcagaggca caccaggaga aaagatcggc gcccaggtct gccagtggat tgctttcagc
      421 atcgccatcg ccctgctgac attctacggc ttcagcgcct ggaaggccac ttgcggttgg
      481 gaggaggtct acgtctgttg cgtcgaggtg ctgttcgtga ccctggagat cttcaaggag
      541 ttcagcagcc ccgccacagt gtacctgtct accggcaacc acgcctattg cctgcgctac
      601 ttcgagtggc tgctgtcttg ccccgtgatc ctgatcaagc tgagcaacct gagcggcctg
      661 aagaacgact acagcaagcg gaccatgggc ctgatcgtgt cttgcgtggg aatgatcgtg
      721 ttcggcatgg ccgcaggact ggctaccgat tggctcaagt ggctgctgta tatcgtgtct
      781 tgcatctacg gcggctacat gtacttccag gccgccaagt gctacgtgga agccaaccac
      841 agcgtgccta aaggccattg cc
//
