LOCUS       sequence-94058-e        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for Chrimson-tdTomato in a floxed/forward-reading,
            Cre-dependent manner (Cre turns gene off).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pCAG-FLEX-fwd[Chrimson-tdT]
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    87..120
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    191..224
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag
       61 tgcggccgcg aacctcttcg agggacataa cttcgtataa tgtatgctat acgaagttat
      121 gggtcgatgg tgagatctgg actagagggt cgatggtgat gcttggatca aaataggaag
      181 aatcccaatg ataacttcgt ataaagtatc ctatacgaag ttatactagg tacctaggct
      241 tacttataca gctcatccat gccgtacaga aacagatggt ggcgtccttc agacctctca
      301 tactgttcca caatggtgta gtcttcattg tgggatgtga tatccagctt ggtgtccaca
      361 tagtaatagc cgggcagctg cactggtttc ttggccatgt agatggtttt gaactccacc
      421 aggtagtggc cgccgtcttt cagcttcagg gcctggtgga tttcgccctt cagcactccg
      481 tctctaggat acagccgttc agtgctggcc tcccacccca ttgttttctt ctgcatcacg
      541 ggcccatcgg gtggaaaatt ggtgccgcgc attttgactt tgtagatcag agtgccatcc
      601 tgcagagagg agtcctgtgt cacggtcacc aggcctccat cttcaaaatt catcactctc
      661 tcccacttaa agccctctgg gaaagacagt ttcttgtaat caggaatgtc agcgggatgc
      721 ttgacatagg ccttacttcc gtacataaac tgaggagaca ggatatccca agcgaaaggc
      781 agtggtcctc ccttggtcac tttcagcttg gcagtctgtg tgccctcgta aggcctccct
      841 tcgccctctc cttcaatctc aaattcgtgc ccattcatgc tgccctccat gcgcacctta
      901 aacctcatga actctttgat cacagccata ttgttatcct cgcttgaggc ggttccacta
      961 gaccctgagc cggtacttcc agtcccatgg cccaggaaca gatggtgtct cccctcgc
//
