LOCUS       sequence-9407-ex       10593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pFRT/TO/FLAG/HA-DEST TNRC6B
FEATURES             Location/Qualifiers
     source          1..10593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..42
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(86..105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        277..656
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        657..860
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     811..831
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    862..880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    883..901
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     911..935
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1035..1058
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1059..1085
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1101..1125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6347..6371)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6424..6465
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6545..6562)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6551..6775
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6908..6927)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7059..7106
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7114..8136
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7114..8136
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8266..8387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8303..8322)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8357..8376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8436..8452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8436..8452)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8449..8471)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8460..8476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8484..8490,8491..8508,8509..8514))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8529..8550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8667..8684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8838..9426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8918..8937)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9597..10388,10389..10457))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10220..10239
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10458..10562)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cggacggatc gggagatctc
       61 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
      121 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
      181 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      241 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      301 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      361 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      421 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      481 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      541 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      601 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      661 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      721 gtctccaccc cattgacgtc aatgggagtt tgttttggaa ccaaaatcaa cgggactttc
      781 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      841 aggtctatat aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag
      901 atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      961 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
     1021 agcttggtac catggactac aaggacgacg atgacaagta cccttatgac gtgcccgatt
     1081 acgctttgaa ccagatatca acaagtttgt acaaaaaagc aggctccacc atgggaacca
     1141 attcagtcga catgagagag aaggagcaag aaagggaaga acagttaatg gaagacaaga
     1201 aaaggaagaa agaggataaa aagaaaaaag aagccactca gaaggtcacg gaacaaaaaa
     1261 ccaaagtgcc cgaagtgacg aaaccaagtt taagccaacc aacggccgcc agcccaattg
     1321 gcagctctcc atcgccacca gtcaatggtg gcaacaatgc caaaagggtg gcagtgccga
     1381 acggacaacc gccaagcgcc gcccgctaca tgcctcggga ggtgccgccg cgattccgtt
     1441 gccagcagga ccacaaagtg ttactaaaac gtgggcagcc ccctccaccg tcctgcatgc
     1501 tccttggggg tggggcaggg cctcctccct gcacagcacc tggagcaaac ccaaacaacg
     1561 cacaagtgac aggagcgctg ctgcagagtg agagtgggac tgcgccagac tcaacccttg
     1621 gaggtgctgc tgcttcaaat tatgcaaatt ccacttgggg ctcgggagcc tcctccaaca
     1681 acggcacctc ccccaaccca attcacatct gggacaaggt gattgtagac gggtctgaca
     1741 tggaagagtg gccttgtatt gccagcaaag acactgaatc ttcttccgaa aacaccaccg
     1801 ataacaacag tgcctcgaac cctggctctg agaagagcac tctgccagga agcaccacta
     1861 gtaacaaagg aaaagggagc cagtgccagt ctgcaagttc tgggaacgaa tgtaatcttg
     1921 gggtctggaa atctgaccct aaggctaaat ctgttcaatc ttccaactct actacagaga
     1981 acaacaatgg actaggaaat tggaggaatg tgagtggtca ggatagaatt ggacctggct
     2041 ctggcttcag caactttaac ccaaatagca acccatctgc ctggccagca ctggtccaag
     2101 aaggaacttc taggaaaggg gcattggaaa cagataatag taattccagt gcacaggtta
     2161 gcacagtagg tcagacatcc agggaacagc agtcaaagat ggaaaatgcg ggtgttaatt
     2221 ttgttgtctc tggcagagaa caggctcaaa ttcataacac tgatggacca aaaaatggaa
     2281 acactaactc cttgaactta agttcaccaa accccatgga gaataaggga atgccctttg
     2341 gaatgggctt ggggaacacc tccaggagca ctgatgcccc ttcacaaagc actggagatc
     2401 gaaagactgg gagtgttgga tcttggggtg cagctagggg gccttctgga actgacacag
     2461 tctctggaca aagcaattct ggaaacaatg ggaacaatgg aaaagagaga gaggactcct
     2521 ggaaaggagc ttctgttcag aaatcaactg ggtcaaaaaa tgactcttgg gacaacaata
     2581 acaggtctac gggtgggtcc tggaactttg gcccccagga ctctaatgac aacaaatggg
     2641 gtgaagggaa caaaatgaca tctggggtct ctcagggaga atggaaacag ccgactgggt
     2701 ctgatgagtt gaaaattgga gaatggagtg gtccaaacca accaaattct agcactggag
     2761 catgggacaa tcaaaagggc caccccctcc ctgaaaacca aggcaatgcc caggctccct
     2821 gttggggaag atcttccagc tccacaggaa gtgaagttgg aggtcaaagc actggaagca
     2881 accacaaagc aggaagtagt gacagtcata actctggccg tcggtcgtac aggcccacac
     2941 atcctgattg tcaggctgtc ttgcagactc ttttgagccg aactgatttg gaccccaggg
     3001 tgctctcaaa cactggctgg ggccaaactc aaattaagca ggacacagtg tgggacattg
     3061 aagaggtgcc aaggcctgag gggaaatctg acaaaggaac tgaggggtgg gagagcgctg
     3121 ccacacagac caagaactca gggggctggg gagatgcacc cagccaaagc aatcaaatga
     3181 agtctggatg gggggagctc tcagcctcta cagagtggaa agaccccaag aacacaggag
     3241 gctggaatga ctacaagaac aacaactctt ccaactgggg aggaggacga cctgatgaaa
     3301 agacaccttc ctcttggaat gagaatccca gcaaggatca ggggtgggga ggtggacgcc
     3361 agcccaatca aggatggtct tctggaaaga atggttgggg ggaggaagtc gatcagacaa
     3421 aaaacagcaa ttgggaaagt tctgcaagta aacctgtgtc tgggtggggt gaaggagggc
     3481 agaatgaaat cgggacttgg ggtaatggtg gcaatgcaag cctagcttca aaaggtgggt
     3541 gggaggattg caaaagatcc ccagcatgga atgagacggg ccgacagccc aattcctgga
     3601 ataaacaaca ccaacagcag cagcccccac agcagccgcc gccaccacaa ccagaggctt
     3661 ctggttcgtg gggaggccca cccccaccac ctccaggcaa cgttcgacct tccaattcca
     3721 gctggagcag cgggccacag cctgcaacac ctaaggatga ggaacccagt ggttgggaag
     3781 agccatcccc acagtcaatt agtcggaaaa tggacattga tgatggcact tcagcatggg
     3841 gagaccctaa cagttataac tacaagaatg tgaatctgtg ggataagaat tcccaagggg
     3901 gcccagcacc tcgagaacca aacctgccca ccccaatgac cagtaaatcg gcatcagatt
     3961 ccaaatctat gcaagacggc tggggggaga gtgacgggcc agtcacagga gctcgccatc
     4021 ccagctggga agaggaggag gatggaggag tctggaacac cactggctct cagggcagtg
     4081 cttcctccca caactcagca agctggggac aaggaggaaa gaaacaaatg aagtgctcac
     4141 tcaaaggagg aaacaatgat tcatggatga atcctcttgc caaacagttt tcaaatatgg
     4201 gattgctgag tcagactgaa gataatccaa gcagcaaaat ggatttgtct gtaggaagcc
     4261 tttcagataa aaaatttgat gtggacaagc gagcgatgaa tctcggggat tttaatgata
     4321 tcatgaggaa ggatcgatct gggttccgtc cacctaattc caaagacatg ggaaccacag
     4381 atagtgggcc ttattttgag aagggcggta gtcatggttt gtttggaaac agcacagcac
     4441 aatcgagagg tctgcacaca cccgtgcagc cactaaattc ttctcccagt ctccgggcgc
     4501 aagtgcctcc ccagtttatt tccccccagg tttctgcctc aatgctcaag cagtttccca
     4561 acagtggcct gagtccaggt cttttcaatg tggggcccca gttatctcct caacaaattg
     4621 ccatgctgag ccagcttcca caaattcccc agtttcagtt ggcatgtcag cttctcttgc
     4681 agcagcagca acagcagcag ttgttacaga accagagaaa gatttctcaa gctgtacgcc
     4741 aacagcaaga gcagcagctg gctcgaatgg tgagtgcact gcagcagcag cagcagcagc
     4801 agcagaggca gccaggcatg aagcactcgc cctctcatcc tgttgggccc aagccgcatc
     4861 tggacaacat ggtacccaac gcattgaatg tggggctccc agaccttcaa accaaagggc
     4921 caatacctgg atatggttct ggcttcagct ctggcggcat ggactatggc atggttggtg
     4981 ggaaggaggc tggaaccgag tctcgcttta aacagtggac ctccatgatg gaggggctgc
     5041 cctctgtagc cacacaggaa gccaatatgc acaaaaatgg cgctatagtg gcccctggta
     5101 aaacccgggg agggtcaccg tacaaccagt ttgatatcat ccctggtgac acactgggtg
     5161 gccatacggg tcctgctggt gatagctggt tacctgccaa atctccacca acaaataaaa
     5221 tcggaagtaa atccagcaat gccagttggc ctccagaatt ccaaccagga gtgccatgga
     5281 aaggtatcca aaacattgac cctgaatctg acccctatgt caccccagga agtgtgctgg
     5341 ggggtacagc cacatctccc attgtagata ctgaccacca actgctgcgg gataacacca
     5401 cagggtctaa ttcttccctc aacacctcgc tgccttcacc tggtgcctgg ccctacagtg
     5461 cctctgacaa ctcctttacc aacgttcata gcacttcagc aaagttccct gattacaaat
     5521 caacatggtc cccagatccc ataggacaca accccactca tctctccaac aagatgtgga
     5581 aaaaccatat ttcctccagg aacactacac cgctgccccg cccacctcct ggtctgacca
     5641 accccaaacc atcatctccc tggagcagca cagcaccccg atcagtcagg gggtggggga
     5701 cacaggactc acggctcgcc tcggcctcta cctggagtga tggtggctca gttcgtccta
     5761 gttactggct ggttcttcac aatctcaccc cacagattga tgggtcaacc ttgagaacga
     5821 tctgcatgca gcatggccca ctgctgacat tccatctgaa tctaacccag ggcactgccc
     5881 tgatccgata cagcaccaaa caggaggcgg ccaaggccca aactgcactg cacatgtgtg
     5941 tgttgggaaa cactaccatc cttgctgagt ttgccactga tgatgaagtc agccgctttc
     6001 tggcacaagc tcagccccct acacctgcag caaccccaag tgcgccagct gcggggtggc
     6061 agtcgctgga gaccggccag aaccagtcag atcccgtggg acctgctctg aatctttttg
     6121 gtgggtccac tgggctcggg cagtggagca gcagcgctgg tggcagcagc ggggccgatc
     6181 ttgctggcgc ttcattgtgg gggcccccaa actattcttc tagcttatgg ggagtcccaa
     6241 cggtggaaga tccccatagg atgggcagcc ctgctccttt actacctggt gaccttctgg
     6301 gaggagggtc ggattcaatc tgagcggccg cactcgagat atctagaccc agctttcttg
     6361 tacaaagtgg ttgatatcca gcacagtggc ggccgctcaa gtctagaggg cccgcggttc
     6421 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggatccgcg
     6481 gccgcataga taactgatcc agtgtgcctc gagtctagag ggcccgttta aacccgctga
     6541 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6601 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6661 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6721 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6781 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6841 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6901 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6961 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7021 cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc
     7081 tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct
     7141 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     7201 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     7261 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     7321 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     7381 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     7441 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     7501 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     7561 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     7621 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     7681 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     7741 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     7801 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     7861 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     7921 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     7981 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     8041 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     8101 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc
     8161 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     8221 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     8281 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8341 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     8401 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     8461 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8521 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8581 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8641 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8701 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8761 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8821 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8881 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8941 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9001 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9061 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9121 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9181 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9241 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     9301 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9361 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9421 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9481 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9541 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9601 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9661 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9721 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9781 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9841 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9901 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9961 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10021 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10081 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10141 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10201 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10261 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10321 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10381 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10441 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10501 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10561 cgcacatttc cccgaaaagt gccacctgac gtc
//
