LOCUS       sequence-9408-ex       10494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pFRT/TO/FLAG/HA-DEST TNRC6C
FEATURES             Location/Qualifiers
     source          1..10494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..42
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(86..105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        277..656
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        657..860
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     811..831
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    862..880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    883..901
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     911..935
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1035..1058
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1059..1085
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1101..1125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3431..3448
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(6248..6272)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6325..6366
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6446..6463)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6452..6676
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6809..6828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6960..7007
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7015..8037
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7015..8037
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8167..8288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8204..8223)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8258..8277
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8337..8353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8337..8353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8350..8372)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8361..8377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8385..8391,8392..8409,8410..8415))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8430..8451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8568..8585)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8739..9327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8819..8838)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9498..10289,10290..10358))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10121..10140
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10359..10463)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cggacggatc gggagatctc
       61 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
      121 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
      181 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      241 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      301 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      361 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      421 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      481 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      541 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      601 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      661 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      721 gtctccaccc cattgacgtc aatgggagtt tgttttggaa ccaaaatcaa cgggactttc
      781 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      841 aggtctatat aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag
      901 atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      961 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
     1021 agcttggtac catggactac aaggacgacg atgacaagta cccttatgac gtgcccgatt
     1081 acgctttgaa ccagatatca acaagtttgt acaaaaaagc aggctccacc atgggaacca
     1141 attcagtcga catggctaca gggagtgccc agggcaactt cactggacat accaagaaga
     1201 caaatggcaa taatggcacc aatggcgcac tcgtccaaag cccttctaat cagagtgccc
     1261 ttggagcagg gggagcgaac agtaatggaa gtgcggccag agtgtggggt gtagccacag
     1321 gctccagctc tggcctggct cactgctctg tcagtggtgg ggatggaaaa atggacacta
     1381 tgattggaga tgggagaagt cagaattgct ggggtgcttc caactccaat gctggcatta
     1441 atcttaacct taatcctaat gccaacccag ctgcctggcc tgtacttgga catgaaggaa
     1501 ccgtggcgac aggcaaccct tccagtattt gcagtccagt cagtgccata ggtcaaaata
     1561 tgggcaacca gaacgggaac ccaacaggca ctttaggtgc ttggggaaac ttgctgccac
     1621 aagagagcac agaaccacaa acgtccactt ctcagaatgt gtctttcagc gcacaacctc
     1681 agaaccttaa cactgatgga ccaaataaca ctaaccccat gaactcttca cccaacccta
     1741 tcaatgcaat gcagacaaat ggactgccaa actggggcat ggctgttggt atgggggcca
     1801 tcatcccgcc ccacctgcaa ggccttcctg gtgctaatgg atcatcagtt tctcaagtca
     1861 gtgggggcag tgctgaagga ataagcaatt ctgtgtgggg actgtcccca ggtaaccctg
     1921 ccacaggaaa tagcaattct gggttcagtc aggggaatgg agacactgtg aactcagcat
     1981 taagtgctaa acaaaatgga tccagcagtg ctgtgcaaaa ggaaggaagt ggaggaaatg
     2041 cttgggattc aggacctcct gctggtcctg gaatactcgc ctggggaagg ggcagtggca
     2101 acaatggcgt tggtaatatc cattcaggag cttggggcca ccccagccga agcacctcta
     2161 acggtgtgaa tggggaatgg ggaaagcccc caaaccagca ttccaacagt gacatcaatg
     2221 ggaaaggatc aacagggtgg gagagtccta gtgtcaccag ccagaaccct accgtacagc
     2281 ctggtggtga acacatgaac tcctgggcca aagcggcatc ttctggaact acagcaagtg
     2341 aaggaagtag tgatggttct ggcaaccaca atgaaggaag cactgggagg gaaggaacgg
     2401 gagaaggccg aaggcgagat aaagggatta tagaccaagg gcacatccag ttgccaagga
     2461 atgatcttga cccaagagtt ctgtctaata ctggttgggg acagactcct gtaaagcaaa
     2521 acactgcctg ggaatttgaa gaatccccta ggtctgaaag gaaaaatgac aatgggacag
     2581 aggcctgggg ttgtgcagct actcaggctt caaactcagg ggggaagaac gatgggtcca
     2641 tcatgaacag tacaaatacc tcttcagtat ctgggtgggt caacgcgcca cctgccgctg
     2701 tgccagcaaa cacaggttgg ggagacagca acaacaaagc gccaagtggc ccgggggttt
     2761 ggggggactc gataagctct actgctgtta gtactgctgc tgctgccaag agtggccatg
     2821 cttggagtgg ggccgcaaat caggaggaca agtcacccac ctggggtgag cctccaaagc
     2881 ccaaatccca acactgggga gatggacaaa gatcaaatcc agcctggagt gcaggagggg
     2941 gagattgggc agattcatcg tctgtccttg gacacttggg ggatgggaaa aaaaatggat
     3001 ctggatggga tgctgacagt aataggtcag ggtctggttg gaatgacacc acgagatctg
     3061 ggaacagtgg ctggggcaac agcacaaata caaaggccaa tccaggtaca aactgggggg
     3121 agactttaaa acctggcccc caacagaact gggctagcaa accccaagac aacaatgtga
     3181 gtaactgggg aggagctgct tctgtgaaac agacaggaac agggtggatc ggggggccgg
     3241 taccggtcaa acagaaggac agcagtgaag caactggctg ggaagaaccc tctccaccgt
     3301 ccattcgccg caaaatggaa attgatgatg gtacctcagc ttggggggac ccaagcaact
     3361 ataacaataa aactgtaaac atgtgggata gaaacaaccc ggtcatccag agcagtacca
     3421 cgaccaatac caccaccacc accaccacta ccacgagcaa caccacacac agggtcgaga
     3481 cgccgccccc gcaccaggct ggtactcagc tgaatcgatc accgttgctt ggtccaggta
     3541 ggaaagtttc atcaggctgg ggagaaatgc ctaatgttca ctcaaagact gaaaactctt
     3601 ggggagaacc atcctcccct tctaccctgg tggataatgg cacagcagca tgggggaagc
     3661 cacccagcag tggcagcggg tggggagatc accctgccga gccgccggtg gcatttggaa
     3721 gagctggcgc acctgttgct gcctcagccc tgtgcaaacc agcttcaaaa tctatgcaag
     3781 aaggctgggg cagtggtggg gatgaaatga acctcagtac cagccagtgg gaggatgaag
     3841 aaggggacgt gtggaataat gctgcttccc aagaaagcac ctcctcctgc agctcctggg
     3901 ggaacgcccc caaaaaagga cttcaaaagg gcatgaagac gtctggcaag caggatgagg
     3961 cctggatcat gagccggctg atcaaacaac tcacagacat gggcttcccg agagagccag
     4021 ctgaggaggc cttgaagagt aacaatatga atcttgatca ggccatgagc gctctgctgg
     4081 aaaagaaggt ggacgtggac aagcgtgggc tgggagtgac cgaccataat ggaatggccg
     4141 ccaagcccct cggctgccgc ccgccaatct ccaaagagtc ttccgtggac cgccccacct
     4201 ttcttgacaa ggatggcggc ctcgtggaag agcccacgcc ttcaccgttc ttgccttccc
     4261 caagcctgaa gctccccctt tcacacagtg cactccccag tcaggccctg ggtgggattg
     4321 cctccgggct gggcatgcaa aacttgaatt cttctagaca gataccgagt ggcaatctgg
     4381 gtatgtttgg caatagtgga gcagcacaag ccaggaccat gcagcagccg ccacagccac
     4441 cagtgcagcc tcttaactct tcccagccca gtctccgtgc tcaagtgcct cagtttctat
     4501 cccctcaggt tcaagcacag cttttgcagt ttgcagcaaa aaacattggt ctcaaccctg
     4561 cactattaac ctcgccaatt aatcctcaac atatgacgat gttgaaccag ctctatcagc
     4621 tgcagctggc ataccaacgt ttacaaatcc agcagcagat gttacaggcc cagcgtaatg
     4681 tgtccggatc catgagacaa caggagcagc aagttgcgcg cacaatcact aatctgcagc
     4741 agcagatcca gcagcaccag cgccagctgg cccaggccct gctcgtgaag cagccaccac
     4801 cgccaccgcc cccgccgcac ctgtctctgc acccctctgc aggcaaatcg gccatggaca
     4861 gcttcccctc gcacccacag actcccggcc tacctgacct gcagaccaaa gagcagcagt
     4921 cttcacccaa cacctttgct ccttaccctc tcgctggact gaacccaaac atgaatgtca
     4981 acagcatgga catgaccggt ggcttgtcgg tgaaggaccc atcccagtcc cagtcacgcc
     5041 tcccccagtg gacgcacccc aactccatgg ataacttgcc cagtgccgct tcccccctgg
     5101 agcagaaccc tagcaagcat ggtgctatcc ctggaggtct aagcattggg cctccaggta
     5161 agtcctccat tgatgactcc tatggccggt acgatttaat ccagaacagt gagtcaccag
     5221 ccagtcctcc cgtagctgtt ccccatagct ggtcacgtgc caaatctgac agtgataaaa
     5281 tctcaaatgg ctctagcatc aactggcccc cagaattcca tccgggagtt ccatggaaag
     5341 gactgcagaa tattgaccct gagaatgacc ctgacgtcac tcctggcagt gtccccactg
     5401 ggcctaccat caacaccacc atccaggatg tcaaccgcta cctcctcaag agtggaggta
     5461 aactgtcaga catcaaatcg acgtggtcct ctggccctac ctcccacacg caagcctctc
     5521 tgtctcatga actatggaag gtgcccagaa acagtactgc acccacgagg ccacctccag
     5581 ggttaaccaa tcccaagccc tcctccacct ggggtgccag ccccctcggc tggaccagct
     5641 cctactcctc gggttctgcc tggagcaccg acacctcagg aagaaccagc agctggctcg
     5701 ttcttcgaaa cctcactccc cagattgatg gttctacact gcggacattg tgtttgcaac
     5761 atgggcctct tatcacattc cacctgaatc tgactcaagg caatgctgtg gtccggtaca
     5821 gctccaagga ggaggctgcc aaggcccaga agtctctgca catgtgcgtc ctgggaaaca
     5881 ctaccatcct ggccgagttc gctggtgaag aagaagtgaa tcgcttctta gcccaaggcc
     5941 aggcgctgcc acccacttcc agctggcagt ccagcagcgc gtccagccag ccgcggctca
     6001 gcgcagcggg cagctcccat ggcctggtac gcagcgacgc tggccactgg aacgccccgt
     6061 gcctgggtgg caaggggagc agtgagctgc tgtggggcgg ggtgccccag tactccagca
     6121 gcctgtgggg cccgcccagc gccgacgaca gcagggtgat aggcagcccc acgccgctaa
     6181 ccaccctgct gcctggggac ctgctcagcg gggagtccct gtaggcggcc gcactcgaga
     6241 tatctagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctca
     6301 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     6361 tctacgcgta ccggatccgc ggccgcatag ataactgatc cagtgtgcct cgagtctaga
     6421 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6481 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6541 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6601 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     6661 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     6721 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6781 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6841 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6901 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
     6961 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
     7021 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     7081 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     7141 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     7201 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     7261 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     7321 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     7381 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     7441 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     7501 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     7561 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     7621 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     7681 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     7741 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     7801 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     7861 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     7921 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     7981 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     8041 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8101 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8161 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8221 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8281 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8341 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8401 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8461 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8521 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8581 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8641 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8701 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8761 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8821 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8881 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8941 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9001 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9061 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9121 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     9181 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9241 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9301 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9361 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9421 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9481 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9541 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9601 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9661 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9721 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9781 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9841 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9901 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9961 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10021 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10081 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10141 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10201 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10261 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10321 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10381 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10441 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
