LOCUS       sequence-94132-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UAS vector to express gene of interest in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Han C, Song Y, Xiao H, Wang D, Franc NC, Jan LY, Jan YN
  TITLE     Epidermal cells are the primary phagocytes in the fragmentation and 
            clearance of degenerating dendrites in Drosophila.
  JOURNAL   Neuron. 2014 Feb 5;81(3):544-60. doi: 10.1016/j.neuron.2013.11.021. 
            Epub 2014 Jan 9.
  PUBMED    24412417
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.11.021"; journalName: "Neuron"; date: 
            "2014-02-5- 5"; volume: "81"; issue: "3"; pages: "544-60"
COMMENT     Sequence Label: pUA
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    90..123
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    175..269
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    293..387
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        406..644
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     591..612
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 gttgtttata tatatcaaaa ataacaacta taataataag aatacattta atttagaaaa
       61 tgcttggatt tcactggaac taggctagca taacttcgta taatgtatgc tatacgaagt
      121 tatgctagcg gatccaagct ggccgcggct cgatccgctt gcatgcctgc aggtcggagt
      181 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      241 gtcctccgag cggagtactg tcctccgagc ggaagcttgc atgcctgcag gtcggagtac
      301 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
      361 cctccgagcg gagtactgtc ctccgagcgg agactctagc gagcgccgga gtataaatag
      421 aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa
      481 gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg
      541 caagttaaag tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg
      601 aaatctgcca agaagtaatt attgaataca agaagagaac tctgaatagg gaattgggaa
      661 ttcgttaaca gatctgcggc cgcggctcga gggtacctct agaggatctt tgtgaaggaa
      721 ccttacttct gtggtgtgac ataatt
//
