LOCUS       sequence-94141-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag FLAG ( not detected by 
            anti GFP ab).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_mRuby2_smFP FLAG
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             31..54
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             586..609
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             616..639
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             652..675
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             682..705
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             898..921
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             925..948
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             952..975
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aggacgacga cgacaaaggt gactacaaag atgatgacga taaaggcgac
       61 tataaggacg atgacgacaa gggcggaaac tcactgatca aggaaaacat gcggatgaag
      121 gtggtgatgg agggctccgt gaatggtcac cagttcaagt gcaccggaga gggagaggga
      181 aacccgtaca tgggaactca gaccatgcgc attaaggtca tcgaaggagg tccgctgccg
      241 ttcgctttcg atatcctggc cacttcgttc ggaggagggt cgcgcacgtt catcaagtac
      301 ccgaagggaa tcccggactt ctttaagcag tcattcccgg aaggattcac ttgggaacgg
      361 gtgacccggt atgaagatgg aggtgtggtg actgtcatgc aagatacttc gctggaggat
      421 gggtgcctcg tgtaccacgt ccaagtccgc ggagtgaatt tcccgtccaa cggaccagtg
      481 atgcagaaaa agacgaaggg ttgggaacct aatactgaaa tgatgtaccc cgcagacgga
      541 gggctgaggg gctacaccca catggcgctg aaggtcgacg gaggagatta caaggatgac
      601 gacgataagc aacaagatta caaagacgat gatgacaagg gccagcaggg cgactacaag
      661 gacgacgacg acaagcagca ggactacaaa gatgacgatg ataaaggagg aggacatctg
      721 tcctgttcgt tcgtgaccac ctacagatca aagaaaaccg tgggaaacat caagatgccg
      781 ggcattcatg ccgtcgacca ccgcctggag cggctcgaag aatcagacaa tgagatgttc
      841 gtcgtgcaaa gagaacatgc cgtggccaag ttcgcgggac tgggaggcgg tggaggcgat
      901 tacaaagacg atgatgacaa gggtgactat aaagacgacg atgacaaagg ggattacaag
      961 gatgatgatg ataagtag
//
