LOCUS       sequence-94146-e         517 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R
  TITLE     Efficient method to generate single-copy transgenic mice by 
            site-specific integration in embryonic stem cells.
  JOURNAL   Genesis. 2006 Jan . 44(1):23-8.
  PUBMED    16400644
REFERENCE   2  (bases 1 to 517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2006 Jan . 44(1):23-8."
COMMENT     Sequence Label: pgk-ATG-frt
FEATURES             Location/Qualifiers
     source          1..517
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    154..275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(191..210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     245..264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(378..402)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(399..437)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    470..488
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cctcactaaa gggaacaaaa gctggtaccg ggccccccct cgagatctag tggatccgat
       61 tttaccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
      121 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
      181 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      241 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgctcgaa gcggccatcg
      301 aattcggggc cgcggaggct ggatcggtcc cggtgtcttc tatggaggtc aaaacagcgt
      361 ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat ataggcctcc
      421 caccgtacac gcctacctcg acccgggtac cgagctcgac tttcactttt ctctatcact
      481 gatagggagt ggtaaactcg actttcactt ttctcta
//
