LOCUS       sequence-94167-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for E. coli expression of Patronin CC truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Hendershott MC, Vale RD
  TITLE     Regulation of microtubule minus-end dynamics by CAMSAPs and 
            Patronin.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):5860-5. doi: 
            10.1073/pnas.1404133111. Epub 2014 Mar 26.
  PUBMED    24706919
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1404133111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-04-22- 22"; volume: "111"; issue: "16"; pages: "5860-5"
COMMENT     Sequence Label: pET-28a-GFP-Patronin CC 868–1457
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..61
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(98..126)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     721..741
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggcggcggc agtggcggcg gcagtggcgg cagtgcctgg agccacccgc agttcgaaaa
       61 aggcagtatg agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      121 agatggtgat gttaatgggc acaaattttc tgtccgtgga gagggtgaag gtgatgctac
      181 aaacggaaaa ctcaccctta aatttatttg cactactgga aaactacctg ttccgtggcc
      241 aacacttgtc actactctga cctatggtgt tcaatgcttt tcccgttatc cggatcacat
      301 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
      361 atctttcaaa gatgacggga cctacaagac gcgtgctgaa gtcaagtttg aaggtgatac
      421 ccttgttaat cgtatcgagt taaagggtat tgattttaaa gaagatggaa acattcttgg
      481 acacaaactc gaatacaact ttaactcaca caatgtatac atcacggcag acaaacaaaa
      541 gaatggaatc aaagctaact tcaaaattcg ccacaacgtt gaagatggtt ccgttcaact
      601 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      661 ccattacctg tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc gtgaccacat
      721 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agc
//
