LOCUS       sequence-94195-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  is a lentiviral vector coding the shRNA si1 (sigfp) and the gene of 
            resistance to puromycin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  TITLE     biG-biS plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psp-161-si1(sigfp)
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(104..203)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(352..372)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        357..686
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      537..672
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gttctgtatg agaccacaga tccccgaacg gcatcaaggt gaacttcaag agagttcacc
       61 ttgatgccgt tctttttgga aaagcttatc gataccgtcg accacccagc tttcttgtac
      121 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
      181 agtcaaaata aaatcattat ttgccatcca gctgcagctc tggcccgtgt ctcaaaatct
      241 ctgatgttac attgcacaag ataaaaatat atcatcatgg taggaatttt aattaattaa
      301 ttaattaatt aattaattaa ttaaattcgc cagcacagtg gtcgaccctg tggaatgtgt
      361 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      421 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
      481 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
      541 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
      601 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
      661 tttttggagg cctaggcttt tgcaaaaagc ttaccatgac cga
//
