LOCUS       sequence-94196-e         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UAS vector to express gene of interest in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Han C, Jan LY, Jan YN
  TITLE     Enhancer-driven membrane markers for analysis of nonautonomous 
            mechanisms reveal neuron-glia interactions in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9673-8. doi: 
            10.1073/pnas.1106386108. Epub 2011 May 23.
  PUBMED    21606367
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1106386108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-06-7- 7"; volume: "108"; issue: "23"; pages: "9673-8"
COMMENT     Sequence Label: pACU
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    159..253
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        272..510
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     457..478
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 taacccttag catgtccgtg gggtttgaat taactcataa tattaattag acgaaattat
       61 ttttaaagtt ttatttttaa taatttgcga gtacgcaaag cttggctgca tccaacgcgt
      121 tgggagctct ccggatccaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg
      181 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
      241 actgtcctcc gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta
      301 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
      361 aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa
      421 tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa
      481 gtaattattg aatacaagaa gagaactctg aatagggaat tgggaattcg ttaacagatc
      541 tgcggccgcg gctcgagggt acctctagag gatctttgtg aaggaacctt acttctgtgg
      601 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa a
//
