LOCUS       sequence-94197-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  is a lentiviral vector coding the shRNA si2 (sip1090) and the gene 
            of resistance to puromycin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  TITLE     biG-biS plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psp-161-si2(sip1090)
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(126..225)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(374..394)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        379..708
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      559..694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(718..737)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cttggatttg ggaatcttat aagttctgta tgagaccaca gatccccgga cacctctcaa
       61 gcatcattca agagatgatg cttgagaggt gtcctttttg gaaaagctta tcgataccgt
      121 cgaccaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
      181 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagc
      241 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
      301 ggtaggaatt ttaattaatt aattaattaa ttaattaatt aattaaattc gccagcacag
      361 tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      421 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      481 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      541 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
      601 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
      661 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaccatg
      721 accgagtaca agcccacgg
//
