LOCUS       sequence-94199-e         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 
            protein. The Cox4 targeting signal is fused to the N-terminus to 
            direct import of Var1. Complements var1 mutations in mtDNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Sanchirico ME, Fox TD, Mason TL
  TITLE     Accumulation of mitochondrially synthesized Saccharomyces cerevisiae
            Cox2p and Cox3p depends on targeting information in untranslated 
            portions of their mRNAs.
  JOURNAL   EMBO J. 1998 Oct 1;17(19):5796-804.
  PUBMED    9755179
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "1998-10-1"; volume: "17(19)"; pages: "5796-804"
COMMENT     Sequence Label: pEVA1
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             156..221
                     /codon_start=1
                     /product="mitochondrial presequence of yeast cytochrome c 
                     oxidase subunit IV (Hurt et al., 1984)"
                     /label=pre-CoxIV
                     /translation="MLSLRQSIRFFKPATRTLCSSR"
     primer_bind     216..232
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     216..232
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttgctatcaa gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct
       61 cgttcccttt cttccttgtt tctttttctg cacaatattt caagctatac caagcataca
      121 atcaaggaat tccatacaaa tagataacaa gcacaatgct ttcactacgt caatctataa
      181 gatttttcaa gccagccaca agaactttgt gtagctctag aactagtgga tccatgaaac
      241 tgaaactgct gaacatgatc ctgtccatga tgaacaaaac caacaacaac aacaacatca
      301 tcatcaacaa caccctggac tccctgatga acaaaaaact gctgctgaaa aacatgctgc
      361 tggacatgaa caacaaaaaa atgaacaaca tgaaacgtat gctgaacaac aacaacatga
      421 acccggctgg tgctaacccg gttgttcacc gtatcggtcc ggctggtaac atcaacaaca
      481 aactgcagca cctgaacaac atgaacaact ggaacaccca gatctacaac tacaacaaaa
      541 acatggaaat catgaacacc atgaacgaca aactgatcaa caaactgctg tacaaaatga
      601 tgaccctgaa gcttaacaac atgaacatca acaaaa
//
