LOCUS       sequence-94209-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His3 GST tagged RBD double mutant p110 alpha T207D, K227A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, 
            Diefenbacher M, Stamp G, Downward J
  TITLE     RAS and RHO families of GTPases directly regulate distinct 
            phosphoinositide 3-kinase isoforms.
  JOURNAL   Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031.
  PUBMED    23706742
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-23- 
            23"; volume: "153"; issue: "5"; pages: "1050-63"
COMMENT     Sequence Label: pBacPAK p110a RBD DM
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..30
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tcggatctgg aagttctgtt ccaggggccc ctaagcttgg atccctgcag gcctcgagtt
       61 cctccacgac catcttcggg tgaactgtgg ggcatccact tgatgccccc acgaatccta
      121 gtggaatgtt tactccccaa tggaatgata gtgactttag aatgcctccg tgaggccaca
      181 ctcgtcacca tcaaacatga actgttcaga gaggccagga aataccctct ccatcagctt
      241 ctgcaagacg aaacttctta cattttcgta agtgtcaccc aagaagcaga aagggaagaa
      301 ttttttgatg aaacaagacg actttgtgac cttcggcttt ttcaaccctt tttaaaagtt
      361 attgaaccag taggcaaccg tgaagaaaag atcctcaatc gagaaattgg ttttgttatt
      421 ggcatgccag tgtgtgaatt tgatatggtt aaagatccag aagtccaaga ctttcgaagg
      481 aacattctga atgtttgcaa agaagctgtg gacctgcggg atctcaactc gcctcatagc
      541 agagcaatgt atgtctaccc tccaaatgtc gagtcttccc cagaactgcc aaagcacatc
      601 tacaacaagt tagataaagg acaaatcata gtggtgattt gggtaatagt ctctccaaac
      661 aacgacaagc agaagtacga tctgaagatc aatcatgact gtgtgccaga gcaagtcatt
      721 gctgaagcca tcagggcaaa agactcggag catgttgttg tcctctgagc agctgaaact
      781 ctgtgtctta gaatatcagg gcaagtatat tctgaaagtg tgtggctgtg acgaatactt
      841 cctggaaaag taccctctga
//
