LOCUS       sequence-94217-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxycycline-inducible expression of 
            wild-type human NAT10 resistant to a siRNA. Confers resistance to 
            puromycin. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Larrieu D, Britton S, Demir M, Rodriguez R, Jackson SP
  TITLE     Chemical inhibition of NAT10 corrects defects of laminopathic cells.
  JOURNAL   Science. 2014 May 2;344(6183):527-32. doi: 10.1126/science.1252651.
  PUBMED    24786082
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-05-2- 2"; volume: "344"; issue: 
            "6183"; pages: "527-32"
COMMENT     Sequence Label: pICE-FLAG-NAT10-siR-WT
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             162..185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      831..840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggcaag cttgcggccg ccgccaccat ggattacaag gatgacgacg
      181 ataagggatc catgcatcgg aaaaaggtgg ataaccgaat ccggattctc attgagaatg
      241 gagtagctga gcggcaaaga tctctctttg ttgtagttgg ggatcgagga aaagatcagg
      301 tggtaatact tcatcacatg ttatccaaag caactgtgaa ggctcggcct tcagtgctgt
      361 ggtgttataa gaaagagctg gggtttagca gtcaccggaa gaaaagaatg cgacagctgc
      421 agaagaaaat aaagaatgga acactgaaca taaagcagga cgaccccttt gaactcttca
      481 tagcagccac aaacattcgc tactgctact acaacgagac ccacaagatc ctgggcaata
      541 ccttcggcat gtgtgtgctg caggattttg aagccttaac tccaaacttg ctggccagga
      601 ctgtagaaac agtggaaggt ggtgggctag tggtcatcct cctacggacc atgaactcac
      661 tcaagcaatt gtacacagtg actatggatg tgcattccag gtacagaact gaggcccatc
      721 aggatgtggt gggaagattt aatgaaaggt ttattctgtc tctggcctct tgtaagaagt
      781 gtctcgtcat tgatgaccag ctcaacatcc tgcccatctc ctcccacgtt gccaccatgg
      841 aggccctgcc tccccagact ccggatgaga gtcttggtcc ttctgatctg gagctga
//
