LOCUS       sequence-94218-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxycycline-inducible expression of 
            G641E mutant of human NAT10 resistant to a siRNA. Confers resistance
            to puromycin. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Larrieu D, Britton S, Demir M, Rodriguez R, Jackson SP
  TITLE     Chemical inhibition of NAT10 corrects defects of laminopathic cells.
  JOURNAL   Science. 2014 May 2;344(6183):527-32. doi: 10.1126/science.1252651.
  PUBMED    24786082
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-05-2- 2"; volume: "344"; issue: 
            "6183"; pages: "527-32"
COMMENT     Sequence Label: pICE-FLAG-NAT10-siR-G641E
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     45..69
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      152..161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             161..184
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      830..839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tatcgtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac
       61 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
      121 cgatccagcc tccggcaagc ttgcggccgc cgccaccatg gattacaagg atgacgacga
      181 taagggatcc atgcatcgga aaaaggtgga taaccgaatc cggattctca ttgagaatgg
      241 agtagctgag cggcaaagat ctctctttgt tgtagttggg gatcgaggaa aagatcaggt
      301 ggtaatactt catcacatgt tatccaaagc aactgtgaag gctcggcctt cagtgctgtg
      361 gtgttataag aaagagctgg ggtttagcag tcaccggaag aaaagaatgc gacagctgca
      421 gaagaaaata aagaatggaa cactgaacat aaagcaggac gacccctttg aactcttcat
      481 agcagccaca aacattcgct actgctacta caacgagacc cacaagatcc tgggcaatac
      541 cttcggcatg tgtgtgctgc aggattttga agccttaact ccaaacttgc tggccaggac
      601 tgtagaaaca gtggaaggtg gtgggctagt ggtcatcctc ctacggacca tgaactcact
      661 caagcaattg tacacagtga ctatggatgt gcattccagg tacagaactg aggcccatca
      721 ggatgtggtg ggaagattta atgaaaggtt tattctgtct ctggcctctt gtaagaagtg
      781 tctcgtcatt gatgaccagc tcaacatcct gcccatctcc tcccacgttg ccaccatgga
      841 ggccctgcct ccccagactc cggatgagag tcttggtcct tctgatctgg agctga
//
