LOCUS       sequence-9423-ex       11100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Liu B, Paton JF, Kasparov S
  TITLE     Viral vectors based on bidirectional cell-specific mammalian 
            promoters and transcriptional amplification strategy for use in 
            vitro and in vivo.
  JOURNAL   BMC Biotechnol. 2008 May 16;8:49. doi: 10.1186/1472-6750-8-49.
  PUBMED    18485188
REFERENCE   2  (bases 1 to 11100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2008-05-16"; volume: "8:49. doi"; pages: " 
            10.1186/1472-6750-8-49"
COMMENT     Sequence Label: pTYF-mCMV/SYN-EGFP
FEATURES             Location/Qualifiers
     source          1..11100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    75..196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(86..105)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     140..159
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(439..981)
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             complement(1045..1485)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(1522..1540)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1586..1624)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        1783..2230
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2281..2290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2287..2289,2290..2292,2293..3003)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2287..2289,2290..2292,2293..3003)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2332..2353)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2593..2612)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2940..2961
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3004..3069
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(3196..3216)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3614..3625)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3928..4152
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4285..4304)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(5325..5342)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5496..6084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5576..5595)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6255..7046,7047..7115))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6878..6897
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7116..7220)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7288..7306
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7357..7379)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7465..7603
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7518..7537
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8807..8827
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    10448..10681
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggcctaggga taacagggta atgatatctc tgaccgctag catcgtcgac ccgggatccg
       61 cggactagtc gcgttaagat acattgatga gtttggacaa accacaacta gaatgcagtg
      121 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
      181 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
      241 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
      301 tcatgaacag actgtgagga ctgaggggcc tgaaatgagc cttgggactg tgaatctaaa
      361 atacacaaac aattagaatc agtagtttaa cacattatac acttaaaaat tggatctact
      421 tattcaagat cggccgctgg agctgatctg actcagcaga gcagagaagt ccatgtccgc
      481 aatggaggag aagtcttcat ctccggagag accattggga agccccgagg tccccagggg
      541 tgtgggagct gggtcagggg gcctctggga ccctgtcacc aggcgagtta tagcttcagg
      601 gtactccatc agcatgggct cagctgtgga gtgagacatg gacacaccct ggttcaggag
      661 ctgctgaaac tctgagttgt ccacagatgc caggtctgtg aacactcctg ggtctgtgct
      721 gttgccaagc aaggccccca agtcttcatc agcatcaaac tgcaggtgca gcagggcttc
      781 cgacagcgtg ccttccccag cctgggtgct ctttggaaca ggtgcagaca gggactgggg
      841 aggacccggg gttagaactg gggcaggagc tgggggctga gccagaggta ccatggctga
      901 ggaagggacc atggtctggg caaggactgg ggcagaggac ggtgctaagg ccagggcctg
      961 gtttgagatc tgcccagaag gaaacaccat ggtgagctct gcagaagctt ctagaattcc
     1021 agcccgggcg gatcccaatt ccggcgatac agtcaactgt ctttgacctt tgttactact
     1081 ctcttccgat gatgatgtcg cacttattct atgctgtctc aatgttagag gcatatcagt
     1141 ctccactgaa gccaatctat ctgtgacggc atctttattc acattatctt gtacaaataa
     1201 tcctgttaac aatgctttta tatcctgtaa agaatccatt ttcaaaatca tgtcaaggtc
     1261 ttctcgagga aaaatcagta gaaatagctg ttccagtctt tctagccttg attccacttc
     1321 tgtcagatgt gccctagtca gcggagacct tttggttttg ggagagtagc gacactccca
     1381 gttgttcttc agacacttgg cgcacttcgg tttttctttg gagcacttga gctttttaag
     1441 tcggcaaata tcgcatgctt gttcgataga agacagtagc ttcatctttc aggaggctag
     1501 cttagatccc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcgct
     1561 agcggatctg acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc
     1621 taacgcgcta gccttgcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc
     1681 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag actctagagg
     1741 gacgcgtatc gatggcgcca gctgcagagg gccctgcgta tgagtgcaag tgggttttag
     1801 gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc cactggacaa
     1861 gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag
     1921 gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc
     1981 gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg
     2041 tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc
     2101 cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg
     2161 cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct
     2221 gagagcgcag tcgactctag agctgcaggc aagcttggta ccgagctcgg atccactagt
     2281 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2341 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2401 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2461 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     2521 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2581 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2641 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2701 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     2761 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     2821 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2881 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     2941 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3001 aagtccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
     3061 ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacgcg gccgctcccg
     3121 ttatttgcgc tctgttcctg ttaatcaacc tctggattac aaaatttgtg aaagattgac
     3181 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3241 gtatcatgct attacttccc gtacggcttt cattttctcc tccttgtata aatcctggtt
     3301 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3361 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctatcaac tcctttccgg
     3421 gactttcgct ttccccctcc ctattgccac ggcggaactc attgccgcct gccttgcccg
     3481 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     3541 gacgtccttt ccatggctgc tcgcctgtgt tgccaactgg attctgcgcg ggacgtcctt
     3601 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggt
     3661 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3721 cgcctatcga ttagggataa cagggtaatg gtacctttaa gaccaatgac ttacaaggca
     3781 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     3841 cagggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3901 cactgcttaa gcctcaataa agctcgactg tgccttctag ttgccagcca tctgttgttt
     3961 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4021 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4081 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4141 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4201 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4261 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4321 tcgccggctg tgcgggagaa cggagttcta ttatgactca aatcagtctc cccaagcatt
     4381 cggggatcag agtttttaag gataacttag tgtgtagggg gccagtgagt tggagatgaa
     4441 agcgtaggga gtcgaaggtg tccttttgcg ccgagtcagt tcctgggtgg gggccacaag
     4501 atcggatgag ccagtttatc aatccggggg tgccagctga tccatggagt gcagggtctg
     4561 caaaatatct caagcactga ttgatcttag gttttacaat agtgatgtta ccccaggaac
     4621 aatttgggga aggtcagaat cttgtagcct gtagctgcat gactcctaaa ccataatttc
     4681 ttttttgttt tttttttttt atttttgaga cagggtctca ctctgtcacc taggctggag
     4741 tgcagtggtg caatcacagc tcactgcagc ctcaacgtcg taagctcaag cgatcctccc
     4801 acctcagcct gcctggtagc tgagactaca agcgacgccc cagttaattt ttgtattttt
     4861 ggtagaggca gcgttttgcc gtgtggccct ggctggtctc gaactcctgg gctcaagtga
     4921 tccagcctca gcctcccaaa gtgctgggac aaccggggcc agtcactgca cctggcccta
     4981 aaccataatt tctaatcttt tggctaattt gttagtccta caaaggcagt ctagtcccca
     5041 ggcaaaaagg gggtttgttt cgggaaaggg ctgttactgt ctttgtttca aactataaac
     5101 taagttcctc ctaaacttag ttcggcctac acccaggaat gaacaaggag agcttggagg
     5161 ttagaagcac gatggaattg gttaggtcag atctctttca ctgtctgagt tataattttg
     5221 caatggtggt tcaaagactg cccgcttctg acaccagtcg ctgcattaat gaatcggcca
     5281 acgcgcgggg agaggcggtt tgcgtattgg cgctcttccg cttcctcgct cactgactcg
     5341 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5401 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5461 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5521 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5581 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5641 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     5701 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5761 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5821 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5881 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     5941 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6001 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6061 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6121 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6181 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6241 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6301 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6361 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6421 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6481 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6541 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6601 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6661 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6721 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6781 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6841 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6901 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6961 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7021 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7081 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7141 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7201 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     7261 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     7321 gaactgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     7381 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     7441 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     7501 gtactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     7561 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     7621 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcgcgg
     7681 ggaggcagag attgcagtaa gctgagatcg cagcactgca ctccagcctg ggcgacagag
     7741 taagactctg tctcaaaaat aaaataaata aatcaatcag atattccaat cttttccttt
     7801 atttatttat ttattttcta ttttggaaac acagtccttc cttattccag aattacacat
     7861 atattctatt tttctttata tgctccagtt ttttttagac cttcacctga aatgtgtgta
     7921 tacaaaatct aggccagtcc agcagagcct aaaggtaaaa aataaaataa taaaaaataa
     7981 ataaaatcta gctcactcct tcacatcaaa atggagatac agctgttagc attaaatacc
     8041 aaataaccca tcttgtcctc aataatttta agcgcctctc tccaccacat ctaactcctg
     8101 tcaaaggcat gtgccccttc cgggcgctct gctgtgctgc caaccaactg gcatgtggac
     8161 tctgcagggt ccctaactgc caagccccac agtgtgccct gaggctgccc cttccttcta
     8221 gcggctgccc ccactcggct ttgctttccc tagtttcagt tacttgcgtt cagccaaggt
     8281 ctgaaactag gtgcgcacag agcggtaaga ctgcgagaga aagagaccag ctttacaggg
     8341 ggtttatcac agtgcaccct gacagtcgtc agcctcacag ggggtttatc acattgcacc
     8401 ctgacagtcg tcagcctcac agggggttta tcacagtgca cccttacaat cattccattt
     8461 gattcacaat ttttttagtc tctactgtgc ctaacttgta agttaaattt gatcagaggt
     8521 gtgttcccag aggggaaaac agtatataca gggttcagta ctatcgcatt tcagggtggc
     8581 ccgagagctg catccggagt atctagatgg agttccgcgt tacataactt acggtaaatg
     8641 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg actgatgttc
     8701 ccatagtaac gccaataggg actttccatt gacgctaatg ggagtttgtt ttggcaccaa
     8761 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     8821 aggcgtgtac tctagaaggt ctatataagc agagctcgtt tagtgaaccg tttatactac
     8881 ttatctggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8941 acccactgct taagcctcaa taaagcttgc cttgagtgct caaagtagtg tgtgcccgtc
     9001 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9061 ctagcagtgg cgcccgaaca gggacttgaa agcgaaagta aagccagagg agatctctcg
     9121 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     9181 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcgg
     9241 tattaagcgg gggagaatta gataaatggg aaaaaattcg gttaaggcca gggggaaaga
     9301 aacaatataa actaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     9361 atcctggcct tttagagaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     9421 cccttcagac aggatcagaa gaacttagat cattatataa tacaatagca gtcctctatt
     9481 gtgtgcatca aaggatagat gtaaaagaca ccaaggaagc cttagataag atagaggaag
     9541 agcaaaacaa aagtaagaaa aaggcacagc aagcagcagc tgacacagga aacaacagcc
     9601 aggtcagcca aaattaccct atagtgcaga acctccaggg gcaaatggta catcaggcca
     9661 tatcacctag aactttaaat gcatgggtaa aagtagtaga agagaaggct ttcagcccag
     9721 aagtaatacc catgttttca gcattatcag aaggagccac cccacaagat ttaaatacca
     9781 tgctaaacac agtgggggga catcaagcag ccatgcaaat gttaaaagag accatcaatg
     9841 aggaagctgc agaatgggat agattgcatc cagtgcatgc agggcctatt gcaccaggcc
     9901 agatgagaga accaagggga agtgacatag caggaactac tagcaaatta agagaacaat
     9961 ttggaaataa taaaacaata atctttaagc aatcctcagg aggggaccca gaaattgtaa
    10021 cgcacagttt taattgtgga ggggaatttt tctactgtaa ttcaacacaa ctgtttaata
    10081 gtacttggtt taatagtact tggagtactg aagggtcaaa taacactgaa ggaagtgaca
    10141 caatcacact cccatgcaga ataaaacaat ttataaacat gtggcaggaa gtaggaaaag
    10201 caatgtatgc ccctcccatc agtggacaaa ttagatgttc atcaaatatt actgggctgc
    10261 tattaacaag agatggtggt aataacaaca atgggtccga gatcttcaga cctggaggag
    10321 gcgatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
    10381 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
    10441 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcac
    10501 ggtcaatgac gctgacggta caggccagac aattattgtc tgatatagtg cagcagcaga
    10561 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
    10621 tcaaacagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
    10681 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
    10741 gttggagtaa taaatctctg gaacagattt ggaataacat gacctggatg gagtgggaca
    10801 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
    10861 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcttgcggc cggaattcac
    10921 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    10981 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    11041 ttactaaaaa ttcnaaaatt ttcgggttta ttacagggac agcagagatc cactttgggc
//
