LOCUS       sequence-94242-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for cloning and expressing your gene of interest. 
            Zeocin selection. This plasmid is not available at Addgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Morgenstern JP, Land H
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990-06-25"; volume: "18(12)"; pages: "3587-96"
COMMENT     Sequence Label: pBABE-zeo (pBABE-bleo)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..436)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(36..52)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(68..90)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    637..836
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 accacactgg gatccggccg gcgcctagag aaggagtgag ggctggataa agggaggatc
       61 gaggcggggt cgaacgagga ggttcaaggg ggagagacgg ggcggatgga ggaagaggag
      121 gcggaggctt agggtgtaca aagggcttga cccagggagg ggggtcaaaa gccaaggctt
      181 cccaggtcac gatgtagggg acctggtctg ggtgtccatg cgggccaggt gaaaagacct
      241 tgatcttaac ctgggtgatg aggtctcggt taaaggtgcc gtctcgcggc catccgacgt
      301 taaaggttgg ccattctgca gagcagaagg taacccaacg tctcttcttg acatctaccg
      361 actggttgtg agcgatccgc tcgacatctt tccagtgacc taaggtcaaa cttaagggag
      421 tggtaacagt ctggccctaa ttttcagaca aatacagaaa cacagtcaga cagagacaac
      481 acagaacgat gctgcagcgc tgcagcagac aagacgcgcg gcttcggttc caaaccgaaa
      541 gcaaaaattc agacggaggc gggaactgtt ttaggttctc gtctcctacc agaaccacat
      601 atcctgacgg ggtcggattc cacatcgact cccttcctca ggtcgggcca caaaaacggc
      661 ccccaaagtc cctgggacgt ctcccagggt tgcggccggg tgttcagaac tcgtcagttc
      721 caccacgggt ccgccagata cagagctagt tagctaacta gtaccgacgc aggcgcataa
      781 aatcagtcat agacactaga caatcggaca gacacagata agttgctggc cagcttgcct
      841 cccggtggtg ggtcggtggt
//
