LOCUS       sequence-94264-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged and mutated Osh2 PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Roy A, Levine TP
  TITLE     Multiple pools of phosphatidylinositol 4-phosphate detected using 
            the pleckstrin homology domain of Osh2p.
  JOURNAL   J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.
  PUBMED    15271978
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-22"; volume: "279(43)"; pages: "44683-9. Epub
            2004 Jul 22"
COMMENT     Sequence Label: pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     270..289
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(584..612)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
       61 ccccctcgag gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
      121 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
      181 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
      241 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
      301 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgatccggtc
      361 accttacttg gcaaggcata tacccatttg ggataagggt aaacatcttt gaattgtcga
      421 aatgaaacgt atataagcgc tgatgttttg ctaagtcgag gttagtatgg cttcatctct
      481 catgagaata agaacaacaa caaatagagc aagcaaattc gagaattcct gcagcccggg
      541 caattcgaat ataatgtcta aaggagaaga acttttcact ggagttgtcc caattcttgt
      601 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      661 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      721 atggccaaca cttgtcacta cttttacgta tggtgttcaa tgcttttcaa gatacccaga
      781 tcatatgaaa cggcatgac
//
