LOCUS       sequence-9427-ex       11496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Liu B, Wang S, Brenner M, Paton JF, Kasparov S
  TITLE     Enhancement of cell-specific transgene expression from a Tet-Off 
            regulatory system using a transcriptional amplification strategy in 
            the rat brain.
  JOURNAL   J Gene Med. 2008 May . 10(5):583-92.
  PUBMED    18324737
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2008 May . 10(5):583-92."
COMMENT     Sequence Label: pTYF-2xGfaABC1D-tTA
FEATURES             Location/Qualifiers
     source          1..11496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        734..752
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             789..1229
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1254..1796
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     primer_bind     complement(1865..1884)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1866..1884)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2158..2279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2195..2214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2249..2268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             join(3092..3712,3713..4099)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3165..3184)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    4324..4548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4681..4700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(5721..5738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5892..6480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5972..5991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6651..7442,7443..7511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7512..7616)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7684..7702
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7753..7775)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7861..7999
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7914..7933
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9203..9223
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    10844..11077
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggcctaggga taacagggta atgattaata acatatcctg gtgtggagta ggggacgctg
       61 ctctgacaga ggctcggggg cctgagctgg ctctgtgagc tggggaggag gcagacagcc
      121 aggccttgtc tgcaagcaga cctggcagca ttgggctggc cgccccccag ggcctcctct
      181 tcatgcccag tgaatgactc accttggcac agacacaatg ttcggggtgg gcacagtgcc
      241 tgcttcccgc cgcaccccag cccccctcaa atgccttccg agaagcccat tgagcagggg
      301 gcttgcattg caccccagcc tgacagcctg gcatcttggg ataaaagcag cacagccccc
      361 taggggctgc ccttgctgtg tggcgccacc ggcggtggag aacaaggctc tattcagcct
      421 gtgcccagga aaggggatca ggggatgccc aggcatggac agtgggtggc agggggggag
      481 aggagggctg tctgcttccc agaagtccaa ggacacaaat gggtgagggg agagctctcc
      541 ccatagctgg gctgcggccc aaccccaccc cctcaggcta tgccaggggg tgttgccagg
      601 ggcacccggg catcgccagt ctagcccact ccttcataaa gccctcgcat cccaggagcg
      661 agcagagcca gagcaggttg gagaggagac gcatcacctc cgctgctcgc gctagcgatt
      721 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctg atctaagcct
      781 cctgaaagat gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa
      841 agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt
      901 gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg
      961 aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg
     1021 acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg
     1081 tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata
     1141 tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta
     1201 acaaaggtca aagacagttg actgtatcgc cggaattggg atccatggtg tttccttctg
     1261 ggcagatctc aaaccaggcc ctggccttag caccgtcctc tgccccagtc cttgcccaga
     1321 ccatggtccc ttcctcagcc atggtacctc tggctcagcc cccagctcct gccccagttc
     1381 taaccccggg tcctccccag tccctgtctg cacctgttcc aaagagcacc caggctgggg
     1441 aaggcacgct gtcggaagcc ctgctgcacc tgcagtttga tgctgatgaa gacttggggg
     1501 ccttgcttgg caacagcaca gacccaggag tgttcacaga cctggcatct gtggacaact
     1561 cagagtttca gcagctcctg aaccagggtg tgtccatgtc tcactccaca gctgagccca
     1621 tgctgatgga gtaccctgaa gctataactc gcctggtgac agggtcccag aggccccctg
     1681 acccagctcc cacacccctg gggacctcgg ggcttcccaa tggtctctcc ggagatgaag
     1741 acttctcctc cattgcggac atggacttct ctgctctgct gagtcagatc agctccagcg
     1801 gccaatctag actgcagctc gacctcgagg gggggcccgg taccaggtaa gtgtacccaa
     1861 ttcgccctat agtgagtcgt attacaattc actcgatcgc ccttcccaac agttgcgcag
     1921 cctgaatggc gaatggagat ccaattttta agtgtataat gtgttaaact actgattcta
     1981 attgtttgtg tattttagat tcacagtccc aaggctcatt tcaggcccct cagtcctcac
     2041 agtctgttca tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2101 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2161 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2221 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     2281 cgcgctagcc ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc
     2341 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact ctagagggac
     2401 gcgtaacata tcctggtgtg gagtagggga cgctgctctg acagaggctc gggggcctga
     2461 gctggctctg tgagctgggg aggaggcaga cagccaggcc ttgtctgcaa gcagacctgg
     2521 cagcattggg ctggccgccc cccagggcct cctcttcatg cccagtgaat gactcacctt
     2581 ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt cccgccgcac cccagccccc
     2641 ctcaaatgcc ttccgagaag cccattgagc agggggcttg cattgcaccc cagcctgaca
     2701 gcctggcatc ttgggataaa agcagcacag ccccctaggg gctgcccttg ctgtgtggcg
     2761 ccaccggcgg tggagaacaa ggctctattc agcctgtgcc caggaaaggg gatcagggga
     2821 tgcccaggca tggacagtgg gtggcagggg gggagaggag ggctgtctgc ttcccagaag
     2881 tccaaggaca caaatgggtg aggggagagc tctccccata gctgggctgc ggcccaaccc
     2941 caccccctca ggctatgcca gggggtgttg ccaggggcac ccgggcatcg ccagtctagc
     3001 ccactccttc ataaagccct cgcatcccag gagcgagcag agccagagca ggttggagag
     3061 gagacgcatc acctccgctg ctcgcggatc catgtctaga ttagataaaa gtaaagtgat
     3121 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     3181 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     3241 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     3301 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     3361 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     3421 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     3481 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     3541 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     3601 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     3661 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag
     3721 ccgcgcgcgt acgaaaaaca attacgggtc taccatcgag ggcctgctcg atctcccgga
     3781 cgacgacgcc cccgaagagg cggggctggc ggctccgcgc ctgtcctttc tccccgcggg
     3841 acacacgcgc agactgtcga cggccccccc gaccgatgtc agcctggggg acgagctcca
     3901 cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga
     3961 catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta
     4021 cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat
     4081 tgacgagtac ggtgggtagc tcgaggctgc agggcggccg catcgattag ggataacagg
     4141 gtaatggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4201 aaagaaaagg ggggactgga agggctaatt cactcccagg gtctctctgg ttagaccaga
     4261 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4321 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4381 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4441 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4501 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     4561 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4621 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4681 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctgtgcg ggagaacgga
     4741 gttctattat gactcaaatc agtctcccca agcattcggg gatcagagtt tttaaggata
     4801 acttagtgtg tagggggcca gtgagttgga gatgaaagcg tagggagtcg aaggtgtcct
     4861 tttgcgccga gtcagttcct gggtgggggc cacaagatcg gatgagccag tttatcaatc
     4921 cgggggtgcc agctgatcca tggagtgcag ggtctgcaaa atatctcaag cactgattga
     4981 tcttaggttt tacaatagtg atgttacccc aggaacaatt tggggaaggt cagaatcttg
     5041 tagcctgtag ctgcatgact cctaaaccat aatttctttt ttgttttttt ttttttattt
     5101 ttgagacagg gtctcactct gtcacctagg ctggagtgca gtggtgcaat cacagctcac
     5161 tgcagcctca acgtcgtaag ctcaagcgat cctcccacct cagcctgcct ggtagctgag
     5221 actacaagcg acgccccagt taatttttgt atttttggta gaggcagcgt tttgccgtgt
     5281 ggccctggct ggtctcgaac tcctgggctc aagtgatcca gcctcagcct cccaaagtgc
     5341 tgggacaacc ggggccagtc actgcacctg gccctaaacc ataatttcta atcttttggc
     5401 taatttgtta gtcctacaaa ggcagtctag tccccaggca aaaagggggt ttgtttcggg
     5461 aaagggctgt tactgtcttt gtttcaaact ataaactaag ttcctcctaa acttagttcg
     5521 gcctacaccc aggaatgaac aaggagagct tggaggttag aagcacgatg gaattggtta
     5581 ggtcagatct ctttcactgt ctgagttata attttgcaat ggtggttcaa agactgcccg
     5641 cttctgacac cagtcgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5701 tattggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5821 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5881 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5941 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6001 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6061 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     6121 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6181 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6241 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6301 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     6361 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6421 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6481 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6541 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6601 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6661 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6721 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6781 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6841 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6901 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6961 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7021 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7081 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7141 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7201 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7261 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7321 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7381 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7441 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7501 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7561 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7621 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     7681 aaaaataggc gtatcacgag gccctttcgt cttcaagaac tgcctcgcgc gtttcggtga
     7741 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     7801 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     7861 cgcagccatg acccagtcac gtagcgatag cggagtgtac tggcttaact atgcggcatc
     7921 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     7981 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     8041 atcggtgcgg gcctcttcgc tattacgcca gcgcggggag gcagagattg cagtaagctg
     8101 agatcgcagc actgcactcc agcctgggcg acagagtaag actctgtctc aaaaataaaa
     8161 taaataaatc aatcagatat tccaatcttt tcctttattt atttatttat tttctatttt
     8221 ggaaacacag tccttcctta ttccagaatt acacatatat tctatttttc tttatatgct
     8281 ccagtttttt ttagaccttc acctgaaatg tgtgtataca aaatctaggc cagtccagca
     8341 gagcctaaag gtaaaaaata aaataataaa aaataaataa aatctagctc actccttcac
     8401 atcaaaatgg agatacagct gttagcatta aataccaaat aacccatctt gtcctcaata
     8461 attttaagcg cctctctcca ccacatctaa ctcctgtcaa aggcatgtgc cccttccggg
     8521 cgctctgctg tgctgccaac caactggcat gtggactctg cagggtccct aactgccaag
     8581 ccccacagtg tgccctgagg ctgccccttc cttctagcgg ctgcccccac tcggctttgc
     8641 tttccctagt ttcagttact tgcgttcagc caaggtctga aactaggtgc gcacagagcg
     8701 gtaagactgc gagagaaaga gaccagcttt acagggggtt tatcacagtg caccctgaca
     8761 gtcgtcagcc tcacaggggg tttatcacat tgcaccctga cagtcgtcag cctcacaggg
     8821 ggtttatcac agtgcaccct tacaatcatt ccatttgatt cacaattttt ttagtctcta
     8881 ctgtgcctaa cttgtaagtt aaatttgatc agaggtgtgt tcccagaggg gaaaacagta
     8941 tatacagggt tcagtactat cgcatttcag ggtggcccga gagctgcatc cggagtatct
     9001 agatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     9061 acccccgccc attgacgtca ataatgactg atgttcccat agtaacgcca atagggactt
     9121 tccattgacg ctaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     9181 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtactcta gaaggtctat
     9241 ataagcagag ctcgtttagt gaaccgttta tactacttat ctggtctctc tggttagacc
     9301 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9361 gcttgccttg agtgctcaaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9421 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     9481 cttgaaagcg aaagtaaagc cagaggagat ctctcgacgc aggactcggc ttgctgaagc
     9541 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     9601 aggctagaag gagagagatg ggtgcgagag cgtcggtatt aagcggggga gaattagata
     9661 aatgggaaaa aattcggtta aggccagggg gaaagaaaca atataaacta aaacatatag
     9721 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctttta gagacatcag
     9781 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     9841 ttagatcatt atataataca atagcagtcc tctattgtgt gcatcaaagg atagatgtaa
     9901 aagacaccaa ggaagcctta gataagatag aggaagagca aaacaaaagt aagaaaaagg
     9961 cacagcaagc agcagctgac acaggaaaca acagccaggt cagccaaaat taccctatag
    10021 tgcagaacct ccaggggcaa atggtacatc aggccatatc acctagaact ttaaatgcat
    10081 gggtaaaagt agtagaagag aaggctttca gcccagaagt aatacccatg ttttcagcat
    10141 tatcagaagg agccacccca caagatttaa ataccatgct aaacacagtg gggggacatc
    10201 aagcagccat gcaaatgtta aaagagacca tcaatgagga agctgcagaa tgggatagat
    10261 tgcatccagt gcatgcaggg cctattgcac caggccagat gagagaacca aggggaagtg
    10321 acatagcagg aactactagc aaattaagag aacaatttgg aaataataaa acaataatct
    10381 ttaagcaatc ctcaggaggg gacccagaaa ttgtaacgca cagttttaat tgtggagggg
    10441 aatttttcta ctgtaattca acacaactgt ttaatagtac ttggtttaat agtacttgga
    10501 gtactgaagg gtcaaataac actgaaggaa gtgacacaat cacactccca tgcagaataa
    10561 aacaatttat aaacatgtgg caggaagtag gaaaagcaat gtatgcccct cccatcagtg
    10621 gacaaattag atgttcatca aatattactg ggctgctatt aacaagagat ggtggtaata
    10681 acaacaatgg gtccgagatc ttcagacctg gaggaggcga tatgagggac aattggagaa
    10741 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    10801 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    10861 ggttcttggg agcagcagga agcactatgg gcgcacggtc aatgacgctg acggtacagg
    10921 ccagacaatt attgtctgat atagtgcagc agcagaacaa tttgctgagg gctattgagg
    10981 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa acagctccag gcaagaatcc
    11041 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    11101 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    11161 agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat tacacaagct
    11221 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    11281 tggaattaga taaatgggct tgcggccgga attcacaaat ggcagtattc atccacaatt
    11341 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    11401 caacagacat acaaactaaa gaattacaaa aacaaattac taaaaattcn aaaattttcg
    11461 ggtttattac agggacagca gagatccact ttgggc
//
