LOCUS       sequence-94272-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  backbone for mouse recombinant antibody with added C-terminal 
            biotinylation site, linker, 6His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Staudt N, Muller-Sienerth N, Fane-Dremucheva A, Yusaf SP, Millrine 
            D, Wright GJ
  TITLE     A panel of recombinant monoclonal antibodies against zebrafish 
            neural receptors and secreted proteins suitable for wholemount 
            immunostaining.
  JOURNAL   Biochem Biophys Res Commun. 2015 Jan 2;456(1):527-33. doi: 
            10.1016/j.bbrc.2014.11.123. Epub 2014 Dec 6.
  PUBMED    25490391
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2014.11.123"; journalName: "Biochem Biophys Res 
            Commun"; date: "2015-01-2- 2"; volume: "456"; issue: "1"; pages: 
            "527-33"
COMMENT     Sequence Label: pVk7-33G1-BioLHis
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..86)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataaatttc ctttattagc cagaggtcga ggtcggggga tccttaatta atcacgtgtc
       61 tagactcaat ggtggtgatg gtggtggggg cccagagggg ccagaggagg caggttcctg
      121 tccctgtggt tccacagcat cttctgggcg tccaggatgt ggtgcaggct gccggaattg
      181 gagccggatt taccaggaga gtgggagagg ctcttctcag tatggtggtt gtgcaggccc
      241 tcatgtaaca cagagcaggt gaaagtattt cctgcctccc agttgctctt ctgcacattg
      301 agcttgctgt agacgaagta agagccatct gtgtccatga tgggctgagt gttcttgtag
      361 ttctccgctg gctgcccatt ccactgccac tccacagtaa tgtcttcagg gaagaagtct
      421 gttatcatgc aggtcagact gactttatcc ttggccatct gctccttggg aggtggaatg
      481 gtgtacacct gtggagcctt cggtctgcct ttggttttgg agatggtttt ctcgatgggg
      541 gcagggaaag ctgcactgtt gaccctgcat ttgaactcct tgccattgag ccagtcctgg
      601 tgcatgatgg gaagttcact gactgagcgg aaagtgctgt tgaactgctc ctcccggggt
      661 tgcgtctgag ctgtgtgcac ctccacatca tctacaaacc agctgaactg gacctcggga
      721 tcatccttgc tgatgtctac cacaacacac gtgacctta
//
