LOCUS       sequence-94273-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged and mutated Osh2 PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Roy A, Levine TP
  TITLE     Multiple pools of phosphatidylinositol 4-phosphate detected using 
            the pleckstrin homology domain of Osh2p.
  JOURNAL   J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.
  PUBMED    15271978
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-22"; volume: "279(43)"; pages: "44683-9. Epub
            2004 Jul 22"
COMMENT     Sequence Label: pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(90..110)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     705..733
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gactcactat agggcgaatt gggtaccaga gctcttaaag ctcatcatgt ttgtatagtt
       61 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
      121 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      181 aaaggacagg gccatcgcca attggagtat tttgttgata atgatcagcg agttgcacgc
      241 cgccgtcttc gatgttgtgg cgggtcttga agttggcttt gatgccgttc ttttgcttgt
      301 cggccatgat gtatacgttg tgggagttgt agttgtattc caacttgtgg ccgaggatgt
      361 ttccgtcctc cttgaaatcg attcccttaa gctcgatcct gttgacgagg gtgtctccct
      421 caaacttgac ttcagcacgt gtcttgtagt tcccgtcgtc cttgaagaag atggtcctct
      481 cctgcacgta tccctcaggc atggcgctct tgaagaagtc gtgccgcttc atatgatcag
      541 ggtaacggga gaagcactgc acgccgtagg tcagggtggt gaccagggtt ggccatggaa
      601 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
      661 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
      721 gaattgggac aactccagtg aaaagttctt ctcctttact cattttttct accggtagag
      781 atccttgtgt tttggaatgc gatatcaggg ctggagattt gttcttcaag gccaatgatg
      841 gtggtactgc c
//
