LOCUS       sequence-94277-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  backbone for mouse recombinant antibody with added C-terminal FLAG 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Staudt N, Muller-Sienerth N, Fane-Dremucheva A, Yusaf SP, Millrine 
            D, Wright GJ
  TITLE     A panel of recombinant monoclonal antibodies against zebrafish 
            neural receptors and secreted proteins suitable for wholemount 
            immunostaining.
  JOURNAL   Biochem Biophys Res Commun. 2015 Jan 2;456(1):527-33. doi: 
            10.1016/j.bbrc.2014.11.123. Epub 2014 Dec 6.
  PUBMED    25490391
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2014.11.123"; journalName: "Biochem Biophys Res 
            Commun"; date: "2015-01-2- 2"; volume: "456"; issue: "1"; pages: 
            "527-33"
COMMENT     Sequence Label: pVk7-33G1-Flag
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..91)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ataaatttcc tttattagcc agaggtcgag gtcgggggat ccttaattaa tcacgtgtct
       61 agatttactt gtcatcgtca tccttgtaat cggagccgga tttaccagga gagtgggaga
      121 ggctcttctc agtatggtgg ttgtgcaggc cctcatgtaa cacagagcag gtgaaagtat
      181 ttcctgcctc ccagttgctc ttctgcacat tgagcttgct gtagacgaag taagagccat
      241 ctgtgtccat gatgggctga gtgttcttgt agttctccgc tggctgccca ttccactgcc
      301 actccacagt aatgtcttca gggaagaagt ctgttatcat gcaggtcaga ctgactttat
      361 ccttggccat ctgctccttg ggaggtggaa tggtgtacac ctgtggagcc ttcggtctgc
      421 ctttggtttt ggagatggtt ttctcgatgg gggcagggaa agctgcactg ttgaccctgc
      481 atttgaactc cttgccattg agccagtcct ggtgcatgat gggaagttca ctgactgagc
      541 ggaaagtgct gttgaactgc tcctcccggg gttgcgtctg agctgtgtgc acctccacat
      601 catctacaaa ccagctgaac tggacctcgg gatcatcctt gctgatgtct accacaacac
      661 acgtgacctt aggagtcaga gtaatggtga gcacatcctt gggctttggg gg
//
