LOCUS       sequence-94278-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to
            drive short RNAs, pgkneo, cassette flanked by loxP sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Brown JR, Zetsche B, Jackson-Grusby L
  TITLE     RUSH and CRUSH: a rapid and conditional RNA interference method in 
            mice.
  JOURNAL   Genesis. 2014 Jan;52(1):39-48. doi: 10.1002/dvg.22718. Epub 2013 Oct
            26.
  PUBMED    24166816
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22718";
            journalName: "Genesis"; date: "2014-01"; volume: "52"; issue: "1"; 
            pages: "39-48"
COMMENT     Sequence Label: pRUSH
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    85..546
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(605..638)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
       61 gacgagttct tctgagggga tccgctgtaa gtctgcagaa attgatgatc tattaaacaa
      121 taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa gggtgagaac
      181 agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg gggtgggatt
      241 agataaatgc ctgctcttta ctgaaggctc tttactattg ctttatgata atgtttcata
      301 gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat tcctcccact
      361 catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg attcccactt
      421 tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa gaacgagatc
      481 agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt tctaattcca
      541 tcagaagctg gtcgaccggt cgagtactag gatccattag gcggccaatt cgtcgaggga
      601 cctaataact tcgtatagca tacattatac gaagttatac atgtttaagg gttccggttc
      661 cactaggtac aattcgatat caagcttatc gataa
//
