LOCUS       sequence-94279-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox 
            conditional U6 promoter pgk-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Brown JR, Zetsche B, Jackson-Grusby L
  TITLE     RUSH and CRUSH: a rapid and conditional RNA interference method in 
            mice.
  JOURNAL   Genesis. 2014 Jan;52(1):39-48. doi: 10.1002/dvg.22718. Epub 2013 Oct
            26.
  PUBMED    24166816
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22718";
            journalName: "Genesis"; date: "2014-01"; volume: "52"; issue: "1"; 
            pages: "39-48"
COMMENT     Sequence Label: pCRUSH
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(591..624)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     650..666
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     651..667
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
       61 cgaaaggagc gcacgacccc atgcatcgat gatatcagat ccccgggatg cagaaattga
      121 tgatctatta aacaataaag atgtccacta aaatggaagt ttttcctgtc atactttgtt
      181 aagaagggtg agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg
      241 gggtggggtg ggattagata aatgcctgct ctttactgaa ggctctttac tattgcttta
      301 tgataatgtt tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag
      361 ctcattcctc ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc
      421 tcttgattcc cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc
      481 ctgaagaacg agatcagcag cctctgttcc acatacactt cattctcagt attgttttgc
      541 caagttctaa ttccatcaga agctggtcga ctctagctag atgcggccgc ataacttcgt
      601 atagtataaa ttatacgaag ttataagcct tgttaacgcg cggtgaccct cgaggtcgac
      661 ggtatcgata agctcgcttc acgagatcat gtttaagggt tccggttcca ctaggtacaa
      721 ttcgatatca agcttatcga taatcaacct ctggattaca aa
//
