LOCUS       sequence-94280-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged and mutated CERT PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Roy A, Levine TP
  TITLE     Multiple pools of phosphatidylinositol 4-phosphate detected using 
            the pleckstrin homology domain of Osh2p.
  JOURNAL   J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.
  PUBMED    15271978
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-22"; volume: "279(43)"; pages: "44683-9. Epub
            2004 Jul 22"
COMMENT     Sequence Label: pRS406-PHO5-GFP-CERT PH domain EE mutation
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(431..460)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(513..533)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gggggatctc tagattcatc
       61 catatccaga ttcagtcttg tgctgttcaa tggcatctat ccattgctgt ctatgatctg
      121 gatcctgagc acgaagatac caaacactat catttacact aatatcaaat cgacattcat
      181 caaaatcgtg aggtgtgatg acagccttgc taagacagat ggatcctctg cagccatact
      241 ctgtttcatc ttcagatttg tagtaactca gagcattatt tttcagtact acccattcat
      301 cctgccaccc atgaatgtag tttgtccact tactgaggac cccgcagcgc tccacaggcg
      361 gcccagactc cgtctctgga tcctcctccg agcccgacga gttccagctc tgattatccg
      421 acatagatct caagtcctct tcagaaatga gcttttgctc catggatcca agcttgtata
      481 gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt
      541 ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg
      601 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
      661 cgcttccatc ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt
      721 tgtctgccat gatgtataca ttgtgtgagt tatagttgta ttccaatttg tgtccaagaa
      781 tgtttccatc ttctttaaaa tcaatacctt ttaactcgat tctattaaca agggtatcac
      841 cttcaaactt gacttcagca cgtgtcttgt ag
//
