LOCUS       sequence-94281-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged and mutated CERT PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Roy A, Levine TP
  TITLE     Multiple pools of phosphatidylinositol 4-phosphate detected using 
            the pleckstrin homology domain of Osh2p.
  JOURNAL   J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.
  PUBMED    15271978
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-22"; volume: "279(43)"; pages: "44683-9. Epub
            2004 Jul 22"
COMMENT     Sequence Label: pRS406-PHO5-GFP-CERT PH domain EE mutation
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..43
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(448..468)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(448..466)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(487..503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(487..503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(500..522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    511..527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(535..541,542..559,560..565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    580..601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(718..735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcttggatcc atggagcaaa agctcatttc tgaagaggac ttgagatcta tgtcggataa
       61 tcagagctgg aactcgtcgg gctcggagga ggatccagag acggagtctg ggccgcctgt
      121 ggagcgctgc ggggtcctca gtaagtggac aaactacatt catgggtggc aggatgaatg
      181 ggtagtactg aaaaataatg ctctgagtta ctacaaatct gaagatgaaa cagagtatgg
      241 ctgcagagga tccatctgtc ttagcaaggc tgtcatcaca cctcacgatt ttgatgaatg
      301 tcgatttgat attagtgtaa atgatagtgt ttggtatctt cgtgctcagg atccagatca
      361 tagacagcaa tggatagatg ccattgaaca gcacaagact gaatctggat atggatgaat
      421 ctagagatcc ccctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
      481 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      541 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      601 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      661 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      721 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactc
//
