LOCUS       sequence-94283-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged and mutated CERT PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Roy A, Levine TP
  TITLE     Multiple pools of phosphatidylinositol 4-phosphate detected using 
            the pleckstrin homology domain of Osh2p.
  JOURNAL   J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.
  PUBMED    15271978
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-22"; volume: "279(43)"; pages: "44683-9. Epub
            2004 Jul 22"
COMMENT     Sequence Label: pRS406-PHO5-GFP-CERT PH domain EE mutation
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     231..250
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(545..573)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacgct ctcccttatg cgactcctgc
       61 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
      121 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
      181 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
      241 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
      301 gcgtagagga tcgatccggt caccttactt ggcaaggcat atacccattt gggataaggg
      361 taaacatctt tgaattgtcg aaatgaaacg tatataagcg ctgatgtttt gctaagtcga
      421 ggttagtatg gcttcatctc tcatgagaat aagaacaaca acaaatagag caagcaaatt
      481 cgagaattcc tgcagcccgg gcaattcgaa tataatgtct aaaggagaag aacttttcac
      541 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      601 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      661 tactggaaaa ctacctgttc catggccaac acttgtcact acttttacgt atggtgttca
      721 atgcttttca agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc
      781 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      841 tgctgaagtc aag
//
