LOCUS       sequence-94284-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 399, Emission:
            511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: mT-Sapphire-pBAD
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..83
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..119
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(123..131,132..146)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(207..228)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(468..487)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     815..836
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actctctact gtttctccat acccgttttt tgggctaaca ggaggaatta accatggggg
       61 gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc
      121 gggatctgta cgacgatgac gataaggatc cgagctcgag catggtgagc aagggcgagg
      181 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      241 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      301 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttctcct
      361 acggcgtgat ggtgttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      421 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      481 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      541 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca
      601 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gccaacttca
      661 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
      721 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc atccagtcca
      781 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      841 ccgcc
//
