LOCUS       sequence-94290-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MSCV based expression vector - expresses GFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   So AY, Sookram R, Chaudhuri AA, Minisandram A, Cheng D, Xie C, Lim 
            EL, Flores YG, Jiang S, Kim JT, Keown C, Ramakrishnan P, Baltimore D
  TITLE     Dual mechanisms by which miR-125b represses IRF4 to induce myeloid 
            and B-cell leukemias.
  JOURNAL   Blood. 2014 Aug 28;124(9):1502-12. doi: 
            10.1182/blood-2014-02-553842. Epub 2014 Jul 8.
  PUBMED    25006123
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2014-02-553842"; journalName: "Blood"; date: 
            "2014-08-28- 28"; volume: "124"; issue: "9"; pages: "1502-12"
COMMENT     Sequence Label: pMG
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(236..255)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     355..377
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(415..433)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        501..605
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(824..843)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaatctttta ttttatcgat aagcttggct gcaggtcgaa attcgttaac ctcgagaaca
       61 gaaatccagg agctgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      121 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
      181 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
      241 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
      301 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
      361 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
      421 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      481 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      541 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      601 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      661 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      721 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      781 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      841 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      901 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg ac
//
