LOCUS       sequence-94294-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MSCV based expression vector - co-expresses HcRed and human 
            miR-125b-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   So AY, Sookram R, Chaudhuri AA, Minisandram A, Cheng D, Xie C, Lim 
            EL, Flores YG, Jiang S, Kim JT, Keown C, Ramakrishnan P, Baltimore D
  TITLE     Dual mechanisms by which miR-125b represses IRF4 to induce myeloid 
            and B-cell leukemias.
  JOURNAL   Blood. 2014 Aug 28;124(9):1502-12. doi: 
            10.1182/blood-2014-02-553842. Epub 2014 Jul 8.
  PUBMED    25006123
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2014-02-553842"; journalName: "Blood"; date: 
            "2014-08-28- 28"; volume: "124"; issue: "9"; pages: "1502-12"
COMMENT     Sequence Label: pHcRed-miR-125b-1
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..741
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
ORIGIN
        1 ccttcactcc ttctctaggc gccggaatta gatccccggg taccggtcgc caccatggtg
       61 agcggcctgc tgaaggagag tatgcgcatc aagatgtaca tggagggcac cgtgaacggc
      121 cactacttca agtgcgaggg cgagggcgac ggcaacccct tcgccggcac ccagagcatg
      181 agaatccacg tgaccgaggg cgcccccctg cccttcgcct tcgacatcct ggccccctgc
      241 tgcgagtacg gcagcaggac cttcgtgcac cacaccgccg agatccccga cttcttcaag
      301 cagagcttcc ccgagggctt cacctgggag agaaccacca cctacgagga cggcggcatc
      361 ctgaccgccc accaggacac cagcctggag ggcaactgcc tgatctacaa ggtgaaggtg
      421 cacggcacca acttccccgc cgacggcccc gtgatgaaga acaagagcgg cggctgggag
      481 cccagcaccg aggtggtgta ccccgagaac ggcgtgctgt gcggccggaa cgtgatggcc
      541 ctgaaggtgg gcgaccggca cctgatctgc caccactaca ccagctaccg gagcaagaag
      601 gccgtgcgcg ccctgaccat gcccggcttc cacttcaccg acatccggct ccagatgctg
      661 cggaagaaga aggacgagta cttcgagctg tacgaggcca gcgtggcccg gtacagcgac
      721 ctgcccgaga aggccaactg agcggccgcg agttttctct gatgtactcg tgatcgtatg
      781 tgtatgtgcg tgattgtata tgcgccccca gatactgcgt atgtgtgtat atatgtatta
      841 ggcttaaacg gaatctcaat tttgtgaagg aaaggagctt agagaag
//
