LOCUS       sequence-94296-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged Opi1 + Terminator in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     Levine plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRS416-PHO5-GFP-Opi1 + terminator
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     260..279
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(574..602)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgccagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag
       61 gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
      121 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
      181 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
      241 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
      301 cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgatccggtc accttacttg
      361 gcaaggcata tacccatttg ggataagggt aaacatcttt gaattgtcga aatgaaacgt
      421 atataagcgc tgatgttttg ctaagtcgag gttagtatgg cttcatctct catgagaata
      481 agaacaacaa caaatagagc aagcaaattc gagaattcct gcagcccggg caattcgaat
      541 ataatgtcta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      601 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      661 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      721 cttgtcacta cttttacgta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa
      781 cggcatgact tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactat
//
