LOCUS       sequence-94307-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged Opi1 + Terminator in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     Levine plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRS416-PHO5-GFP-Opi1 + terminator
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(20..207)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     128..152
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 caggcatttg ctcggcatgc cggtagaggt gtggtcaata agagcgacct catgctatac
       61 ctgagaaagc aacctgacct acaggaaaga gttactcaag aataagaatt ttcgttttaa
      121 aacctaagag tcactttaaa atttgtatac acttattttt tttataactt atttaataat
      181 aaaaatcata aatcataaga aattcgcgag ctcggttagt ccttgctatc cacgttgtcc
      241 tgagagggct ttacgtaatt gccatccttc tgctgttgct gcctgtactg ttgctgctgt
      301 tgcaagaaac gctccctgat catttctttt acctcctgtt tctgcttcac ccattcttca
      361 gccttgccca acgtggagtc gactacgccc atgacatttc tcaccatttc cagggattct
      421 ttggcgagaa tgagtacttt cccattcgca gttacggagg aggacggcga ggctgagtcg
      481 tcgccatcct tgttcctttt ttgctgttgc tgttgcagga gttgctgctg caaaagttgc
      541 tgctgctgct gctgttgctg ttgctgttgc tgttgctcca ctttttgttc ttcacagttg
      601 gcataatgaa acgaagcatg atgcggcagt gatgtactgt ggacgctgtc gaaccaattt
      661 gtgggtaagt ttagaagact ttcccgaacc tgagatcttg cgggctccgg cagcgaattt
      721 gctgtgaact tcgatatcag cgagtagact ttcttgactg tgccgaccac ctccattttc
      781 accacaatag actgctcgct ggcgttgacc tgctctgagg catc
//
