LOCUS       sequence-94309-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 513, Emission:
            527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: YPet-pBAD
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(4..717,718..720,721..723))
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     398..417
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(730..750)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(763..795)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(799..816)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcttactta tagagctcgt tcatgccctc ggtgatgccg gcggcggtca ggaactccag
       61 cagcaccatg tggtcccgct tctcgttggg gtccttgaac agggcgctct ggtagctcag
      121 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      181 gtcggccagc tgcacgccgc cgtcctcgat gttgtgccgg atcttgaagt tggccttgat
      241 gccgttcttc tgcttgtcgg cggtgatgta cacgttgtgg ctgttgtagt tgtactccag
      301 cttgtggccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatccggtt
      361 caccagggtg tcgccctcga acttcacctc ggcccgggtc ttgtagttgc cgtcgtcctt
      421 gaagaagatg gtccgctcct gcacgtagcc ctcgggcatg gcgctcttga agaagtcgtg
      481 ctgcttcatg tggtcggggt accgggcgaa gcactgcacg ccgtagccca gggtggtcac
      541 cagggtgggc cagggcacgg gcagcttgcc ggtggtgcac agcagcttca gggtcagctt
      601 gccgtaggtg gcgtcgccct cgccctcgcc gctcacgctg aacttgtggc cgttcacgtc
      661 gccgtccagc tccaccagga tgggcaccac gccggtgaac agctcttcgc ctttgctcac
      721 catgctcgag ccctggaagt acaggttctc gtacagatcc cgacccattt gctgtccacc
      781 agtcatgcta gccataccat gatgatgatg atgatgagaa ccccgcatat gtatatctcc
//
