LOCUS       sequence-94314-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MSCV based expression vector - co-expresses mCherry and human 
            miR-125b-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   So AY, Sookram R, Chaudhuri AA, Minisandram A, Cheng D, Xie C, Lim 
            EL, Flores YG, Jiang S, Kim JT, Keown C, Ramakrishnan P, Baltimore D
  TITLE     Dual mechanisms by which miR-125b represses IRF4 to induce myeloid 
            and B-cell leukemias.
  JOURNAL   Blood. 2014 Aug 28;124(9):1502-12. doi: 
            10.1182/blood-2014-02-553842. Epub 2014 Jul 8.
  PUBMED    25006123
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2014-02-553842"; journalName: "Blood"; date: 
            "2014-08-28- 28"; volume: "124"; issue: "9"; pages: "1502-12"
COMMENT     Sequence Label: pmCherry-miR-125b-1
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             56..766
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(201..219)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     469..488
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattag atccccgggt accggtgccg ccaccatggt
       61 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
      121 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      181 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
      241 ctgggacatc ctgtcccctc aattcatgta tggctccaag gcctacgtga agcaccccgc
      301 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
      361 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttac aagacggcga
      421 gttcatctac aaagtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
      481 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
      541 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
      601 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
      661 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
      721 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaa
//
